One Function to Rule Them All: An R Shiny Application and R Script for Processing Measurement Data in Microscopic Analysis of Linear Enamel Hypoplasia.

The quantities of data required for microscopic analyses of linear enamel hypoplasia (LEH) require familiarity with software coding to provide objective definitions of enamel defects. Here we provide a pre‐built R script and R Shiny application for identifying accentuated perikymata from microscopic...

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Published in:American Journal of Biological Anthropology Vol. 189; no. 4; pp. 1 - 12
Main Authors: Sellers, Maria L., Temple, Daniel H.
Format: Article
Published: Wiley-Blackwell Apr2026
Subjects:
Online Access:View this record in EBSCOhost
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      dt: Apr2026
      vid: 189
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      pub: Wiley-Blackwell
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        193324468
        10.1002/ajpa.70237
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        atl: One Function to Rule Them All: An R Shiny Application and R Script for Processing Measurement Data in Microscopic Analysis of Linear Enamel Hypoplasia.
      aug:
        au:
          Sellers, Maria L.
          Temple, Daniel H.
        affil:
          University of Florida, Gainesville Florida,, USA
          George Mason University, Fairfax Virginia,, USA
      su:
        Developmental defects of enamel
        Application software
        Computer software
        Standardization
        Data analysis
        Microscopy
      sug:
        subj:
          Custom Computer Programming Services
          Software publishers (except video game publishers)
          Software Publishers
          Computer and software stores
          Computer and Computer Peripheral Equipment and Software Merchant Wholesalers
          Computer, computer peripheral and pre-packaged software merchant wholesalers
          Developmental defects of enamel
          Application software
          Computer software
          Standardization
          Data analysis
          Microscopy
      keyword:
        data processing
        DOHaD
        early life stress
        microscopic LEH
        R
        data processing
        DOHaD
        early life stress
        microscopic LEH
        R
      ab: The quantities of data required for microscopic analyses of linear enamel hypoplasia (LEH) require familiarity with software coding to provide objective definitions of enamel defects. Here we provide a pre‐built R script and R Shiny application for identifying accentuated perikymata from microscopic measurements and calculating the age‐at‐defect by individual and tooth. Our R Shiny application is designed to facilitate analysis based on a method introduced by Bocaege and Hillson (2016) which uses LOWESS residuals objectively define abnormally large perikymata indicative of LEH defects. Example data from the right mandibular first and second incisors from an individual dating to the Middle/Late Colonial period (ca. A.D. 1620–1750) buried at the Chapel of Santa Maria de Magdalena de Eten (CSMME) has been included to demonstrate how the code facilitates LEH analysis. This code represents a move towards further standardization of identifying enamel defects, as well as a means of reducing the time and energy required for analysis on the part of the researcher. This program aims to reduce the risk of error by use of a common code, encourage continued use of these methods for identifying and analyzing LEH, and provide open access to these codes so that future research may build or improve upon these methods.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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