Characterization of pathogenic endosymbionts in free-living amoebae isolated from indoor sport water complexes.
This study aimed to investigate FLA and their endosymbionts in indoor sport water complexes (ISWCs). A total of 90 samples were collected from 15 ISWCs. After morphological and molecular detection, fungi and viral pathogens were investigated in isolated FLA using real-time PCR. Totally, 51 (56.7%) w...
| Publicado en: | International Journal of Environmental Health Research Vol. 36; no. 6; pp. 1502 - 1514 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Taylor & Francis Ltd
Jun2026
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=194221685&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 194221685 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09603123 57L jtl: International Journal of Environmental Health Research issn: 09603123 maglogo: Y pubinfo: dt: Jun2026 vid: 36 iid: 6 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 194221685 188894844 194221685 194221685 10.1080/09603123.2025.2579837 194221685 ppf: 1502 ppct: 12 formats: tig: atl: Characterization of pathogenic endosymbionts in free-living amoebae isolated from indoor sport water complexes. aug: au: Haghbayan, Narjes Niyyati, Maryam Mahdavi, Fatemeh Nemati, Sara Mohebbi, Seyed Reza Zabetpour Kurdi, Hannane Pourhosseingholi, Mohammad Amin Mirjalali, Hamed affil: Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran sug: subj: Protozoa Biological Phenomena Water Microbiology Fungi Viruses Swimming Sports Facilities Funding Source Iran Human Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis Phylogenetics Chi Square Test Fisher's Exact Test Data Analysis Software Descriptive Statistics Biofilms Candida Albicans Aspergillus Water Pollution Sterilization and Disinfection ab: This study aimed to investigate FLA and their endosymbionts in indoor sport water complexes (ISWCs). A total of 90 samples were collected from 15 ISWCs. After morphological and molecular detection, fungi and viral pathogens were investigated in isolated FLA using real-time PCR. Totally, 51 (56.7%) were positive for FLA, including Acanthamoeba sp. Vermamoeba sp. Vahlkampfiidae, and Thecamoeba spp. in 33 (36.7%), 20 (22.2%), 24 (26.7%), and 2 (2.2%) of samples, respectively. The number of positive samples was 17 in each sauna biofilm, Jacuzzi, and pool water. The genotypes T4, T5, and T11 were characterized in Acanthamoeba sp.. All Vermamoeba spp. were V. vermiformis and all family Vahlkampfiidae were characterized as Naegleria sp.. Candida albicans and Aspergillus flavus were detected in two samples of pool water and Jacuzzi. The presence of AdV, NoV, and AsV was recorded in two Jacuzzi samples, in which AdV and NoV were detected in a sample at the same time. Our findings suggest that FLA can resist disinfectant used in ISWCs and high temperature of Jacuzzi. In addition, the presence of viral and fungal endosymbionts contributes to the important role of FLA in waterborne infections, particularly in places where people are in close contact with them. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|