Chronic ethanol exposure reduces resting state functional connectivity and regional synchrony in male rats.

Rationale: Alcohol use disorder (AUD) is a common mental health disorder affecting many individuals and their families in the United States. The effects of alcohol are not fully understood, particularly the effect of alcohol on baseline brain activity. Objectives: We aimed to assess whether chronic...

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Publicado en:Psychopharmacology Vol. 243; no. 5; pp. 1117 - 1130
Autores principales: Crofton, Elizabeth J., Lee, Sung-Ho, Ban, Woomi, Wang, Tzu-Wen Winnie, Shih, Yen-Yu Ian, Morrow, A. Leslie, Herman, Melissa A.
Formato: Journal Article
Publicado: Springer Nature May2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: May2026
      vid: 243
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      pub: Springer Nature
      place: New York, New York
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        194358472
        187425933
        10.1007/s00213-025-06881-0
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        atl: Chronic ethanol exposure reduces resting state functional connectivity and regional synchrony in male rats.
      aug:
        au:
          Crofton, Elizabeth J.
          Lee, Sung-Ho
          Ban, Woomi
          Wang, Tzu-Wen Winnie
          Shih, Yen-Yu Ian
          Morrow, A. Leslie
          Herman, Melissa A.
        affil: https://ror.org/0130frc33 Department of Psychiatry, School of Medicine, University of North Carolina at Chapel Hill, 27599, Chapel Hill, NC, USA
      sug:
      ab: Rationale: Alcohol use disorder (AUD) is a common mental health disorder affecting many individuals and their families in the United States. The effects of alcohol are not fully understood, particularly the effect of alcohol on baseline brain activity. Objectives: We aimed to assess whether chronic ethanol exposure alters resting state functional connectivity between regions of interest (ROIs) previously associated with addiction in male rats. We also aimed to assess whether inhibition of histone deacetylases (HDAC) reduced or blocked the effects of chronic ethanol exposure. Finally, we aimed to investigate whether chronic ethanol exposure altered regional homogeneity (ReHo) and whether HDAC inhibition blocked the effects of ethanol on ReHo. Methods: Male rats were administered water or ethanol (5 g/kg, 25% v/v) via intragastric gavage once daily during the light cycle for 14 days and allowed to withdraw for 24 h. Rats were additionally injected with either the HDAC inhibitor trichostatin A (TSA) (2 mg/kg, i.p.) or vehicle (10% DMSO in 0.9% saline) on the last two days of gavage and on the last day of withdrawal. Rats were scanned with magnetic resonance imaging (MRI) to obtain an anatomical scan as well as resting state functional connectivity (rs-fMRI). Results: We found that chronic ethanol exposure decreased rs-fMRI in the following pairs of ROIs: caudate putamen-prelimbic cortex, caudate putamen-infralimbic cortex, caudate putamen-nucleus accumbens core as well as caudate putamen-insula, insula-prelimbic cortex, and insula-infralimbic cortex. Chronic ethanol exposure also decreased ReHo, particularly in the dorsal striatum. We did not find significant effects of inhibition of HDACs on rs-fMRI of ROIs or ReHo. Conclusions: Chronic alcohol exposure and withdrawal decreases baseline functional connectivity and local synchrony in male rats which is not affected by HDAC inhibition. Future studies should examine the effects of alcohol on resting state connectivity in female rats as well as in voluntary alcohol consumption paradigms. Understanding baseline differences may open new therapeutic avenues in alcohol abuse and AUD to restore typical resting state connectivity.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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