Genetic Surgery: The Revolutionary CRISPR-Cas9 Scissors.
Introduction: The CRISPR-Cas9 system, a revolutionary genome-editing tool which stems from a bacterial adaptive immune system, shows a paradigm shift in designing new therapeutics in medicine. Aim: This review describes Hie CRISPR-Cas9 technology from its initial discovery in prokaryotes as a bacter...
| Publicado en: | Journal of Basic Research in Medical Sciences Vol. 13; no. 1; pp. 67 - 85 |
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| Autores principales: | , |
| Formato: | pictorial review tables/charts Journal Article |
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Ilam University of Medical Sciences
2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=194361506&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 194361506 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23830506 N1MR jtl: Journal of Basic Research in Medical Sciences issn: 23830506 maglogo: N pubinfo: dt: 2026 vid: 13 iid: 1 pid: 63388 pub: Ilam University of Medical Sciences artinfo: ui: 194361506 194361506 194361506 194361506 ppf: 67 ppct: 18 formats: fmt: @attributes: type: P tig: atl: Genetic Surgery: The Revolutionary CRISPR-Cas9 Scissors. aug: au: Alivaisi, Elahe Noori-Zadeh, Ali affil: Ham Univrsity, Department of Biology, Ham, Iran sug: subj: Clustered Regularly Interspaced Short Palindromic Repeats Physiology Gene Editing CRISPR-Associated Proteins Metabolism Genetic Techniques DNA, Recombinant Genetic Variation Immunity, Innate Genetic Engineering Gene Expression Profiling Molecular Biology Drug Delivery Systems Biotechnology Immune System Hereditary Diseases Therapy Immunosuppression ab: Introduction: The CRISPR-Cas9 system, a revolutionary genome-editing tool which stems from a bacterial adaptive immune system, shows a paradigm shift in designing new therapeutics in medicine. Aim: This review describes Hie CRISPR-Cas9 technology from its initial discovery in prokaryotes as a bacterial adaptive immune system to its development into a precise molecular scalpel capable of human genome editing. It explores CRISPR-Cas9 fundamental components and mechanisms, highlighting how a synergy between a guide RNA and Cas9 nuclease allows targeted DNA double-strand breaks, and harness DNA repair enzymes of Hie target cell for genetic code modifications. Discussion: There is a lot of promise for using CRISPR-Cas9 technology to treat diseases. In clinical settings, it has led to breakthroughs in the treatment of monogenic disorders like sickle cell anaemia and beta-Hialassemia, and it is also making good progress in oncology and other areas. The technology still has a lot of problems to solve, even with these successes. For example, it can cause mutations that aren't intended, it has technical limits, and there are moral concerns about how it could be misused, such as for heritable human enhancement. Conclusion: CRISPR-Cas9 technology offers remarkable therapeutic potential with clinical applications already yielding breakthroughs in curing monogenic disorders such as sickle cell anemia and beta-thalassemia, and promising advances in oncology and beyond. However, it also presents significant ethical and practical risks, including the threat of heritable human enhancement (designer babies), and persistent safety concerns such as off-target mutations. pubtype: Academic Journal doctype: pictorial review tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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