Effects of Collagen Hydrolysate-Based Protein Complexes on Physical Endurance, Glucose Metabolism, and Renal Function in Rats.

Background/Objectives: The increasing prevalence of nutrition-related diseases and the limited availability of convenient, metabolically safe, high-protein foods represent a pressing public health challenge. This study aimed to evaluate the effects of four composite animal-derived high-protein ingre...

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Publicado en:Nutrients Vol. 18; no. 11; pp. 1735 - 1759
Autores principales: Kurkin, Denis V., Bakulin, Dmitry A., Osadchenko, Nazar A., Murina, Natalia S., Litvinova, Elena V.
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: MDPI Jun2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jun2026
      vid: 18
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      pub: MDPI
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        10.3390/nu18111735
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        atl: Effects of Collagen Hydrolysate-Based Protein Complexes on Physical Endurance, Glucose Metabolism, and Renal Function in Rats.
      aug:
        au:
          Kurkin, Denis V.
          Bakulin, Dmitry A.
          Osadchenko, Nazar A.
          Murina, Natalia S.
          Litvinova, Elena V.
        affil: Department of Pharmacy, Russian University of Medicine, Moscow 119049, Russia
      sug:
        subj:
          Collagen Pharmacodynamics
          Animal Proteins, Dietary Pharmacodynamics
          Physical Endurance Drug Effects
          Blood Glucose Metabolism
          Blood Glucose Drug Effects
          Kidney Physiology
          Kidney Drug Effects
          Eating Behavior Evaluation
          Carbohydrate Metabolism Drug Effects
          Behavior, Animal Drug Effects
          Dietary Supplementation
          Animal Studies
          Rats
          Funding Source
          Biotransformation
          Cattle
          Swine
          Whey Proteins
          Meat
          Blood Proteins
          Random Assignment
          Animal Proteins, Dietary Administration and Dosage
          Urea Drug Effects
          Urea Blood
          Creatinine Blood
          Creatinine Drug Effects
          Proteins Urine
          Proteins Drug Effects
          Glomerular Filtration Rate Drug Effects
          Physical Endurance Evaluation
          Biological Markers Drug Effects
          Proteinuria Prevention and Control
          Diet, High Protein
          Functional Food
          Swimming
          Fatigue Prevention and Control
          Chromatography, High Pressure Liquid
          Histology
          In Vitro Studies
          Data Analysis Software
          Descriptive Statistics
          One-Way Analysis of Variance
          Post Hoc Analysis
          Comparative Studies
          Biochemistry
      ab: Background/Objectives: The increasing prevalence of nutrition-related diseases and the limited availability of convenient, metabolically safe, high-protein foods represent a pressing public health challenge. This study aimed to evaluate the effects of four composite animal-derived high-protein ingredients based on collagen enzymatic hydrolysates on physical endurance, feeding behaviour, carbohydrate metabolism, renal function, and behavioural parameters in rats. Methods: Four lyophilised collagen hydrolysate-based ingredients were developed using enzymatic biotransformation of bovine and porcine raw materials, combined with bovine whey protein concentrate, bovine meat trim hydrolysate, porcine blood plasma proteins, and an api-component (Samples 1–4; protein content 87–89%). Ninety male Wistar rats were randomised into one control group and four experimental groups (n = 20 per experimental group, n = 10 controls) and received test samples by intragastric gavage at 3000 mg/kg/day for 40 days. Physical endurance was assessed via a weighted forced swimming test (days 0, 30, and 40); behavioural status by open field, adhesive removal, and marble burying tests; and biochemical parameters (blood glucose, serum urea, creatinine, urinary protein, and GFR) at days 0 and 40. Results: All experimental groups demonstrated a significant reduction in standard chow consumption (19–24%, p < 0.01) without affecting body weight gain. Physical endurance improved significantly in all groups relative to baseline, with the most pronounced effect in the Sample 3 group (+39% at day 40, p < 0.05). Blood glucose levels were significantly reduced across all groups (9–16%, p < 0.05). No adverse behavioural effects were observed. Biochemical markers indicated an adaptive rather than pathological renal response, with elevated GFR in three of four experimental groups (p < 0.05) and reduced proteinuria in the Sample 1 and Sample 3 groups. Conclusions: Forty-day administration of collagen hydrolysate-based protein complexes improved physical endurance and glucose metabolism, reduced food intake without compromising body weight, and did not impair renal function or behavioural status in healthy adult rats. These findings support the potential of such ingredients as functional food components, pending confirmation of long-term safety in extended studies.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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