Role of oral yeast in replenishing gastric mucosa with yeast and H. pylori...25th Iranian Congress of Gastroenterology and Hepatology (ICGH), December 16-19, 2025, Tehran, Iran.
Introduction: Relationship between oral and gastric yeasts and their role in colonization of H. pylori in stomach was studied. Materials and Methods: Four groups of 221, 7, 44 and 10 patients were used for isolation of H. pylori and oral and gastric yeasts. In group one, gastric biopsies were used f...
| Publicado en: | Iranian Journal of Gastroenterology & Hepatology (GOVARESH) Vol. 30; pp. 28 - 30 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | abstract proceedings research Journal Article |
| Publicado: |
Iranian Association of Gastroenterology & Hepatology
2025 Supplement
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=194708191&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 194708191 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 15607186 5DI0 jtl: Iranian Journal of Gastroenterology & Hepatology (GOVARESH) issn: 15607186 maglogo: N pubinfo: dt: 2025 Supplement vid: 30 pid: 42050 pub: Iranian Association of Gastroenterology & Hepatology place: , <Blank> artinfo: ui: 194708191 194708191 194708191 194708191 ppf: 28 ppct: 2 formats: tig: atl: Role of oral yeast in replenishing gastric mucosa with yeast and H. pylori...25th Iranian Congress of Gastroenterology and Hepatology (ICGH), December 16-19, 2025, Tehran, Iran. aug: au: Salmanian, Ali-Hatef Sarrafnejad, Abdolfattah Graham, David Y. Hatefi, Atousa Godini, Elham Khalili-Samani, Saman Kadkhodaei, Sara Pedramnia, Shahrzad Siavoshi, Farideh Sotoudeh, Masood Malekzadeh, Reza Saniee, Parastoo affil: Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran. sug: subj: Helicobacter Infections Helicobacter Pylori Candidiasis, Oral Gastric Mucosa Bacterial Colonization Congresses and Conferences Iran Iran ab: Introduction: Relationship between oral and gastric yeasts and their role in colonization of H. pylori in stomach was studied. Materials and Methods: Four groups of 221, 7, 44 and 10 patients were used for isolation of H. pylori and oral and gastric yeasts. In group one, gastric biopsies were used for isolation of H. pylori and yeast, urease activity and staining with Gram's, hematoxylin & eosin (H&E) and immunohistochemistry (IHC) methods. Gram-stained gastric biopsies were examined with light microscope to observe H. pylori, yeast and released extracellular vesicles (EVs). In other 3 groups, DNAs extracted from H. pylori and yeasts were used for amplification of H. pylori-specific genes; 16S rRNA, VacA (s1/s2), vacA (m1/m2), cagA and jhp947.Wet mounts of yeasts in group 2 were examined for observing intracellular bacteria and released EVs. Results: Among 221 patients, 65 (29.3%) had oral yeasts (69.23% C. albicans and 30.76% Candida spp.) followed by H. pylori (35, 15.8%) and gastric yeasts (31, 14%) (67.75% C. albicans and 32.25% Candida spp.) (p value > 0.05). Compared with gastric yeast, culture of oral yeast showed a significant correlation with positive results of H. pylori detected by IHC (10.3%), Gram stain (9%), RUT (6.3%), H&E (4.9%) and culture (4%) (p value < 0.05). Detection of H. pylori genes in yeast isolates from other 3 groups showed that oral and gastric yeasts carried similar H. pylori genes, thus could have a common source. Detection of H. pylori genes in different species of Candida showed that harboring H. pylori is not species- or site- specific. Microscopic examination of Gram-stained gastric biopsies and wet mount preparations of yeasts showed release of EVs that could carry H. pylori. Conclusion: Oral yeast protects its intracellular H. pylori and releases it inside EVs to safely reach gastric mucosa. These results may clarify the environmental reservoir of H. pylori and explain bacterial reinfection after successful eradication. pubtype: Academic Journal doctype: abstract proceedings research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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