Evaluation of the LncRNA TMPO AS-1 Expression Level in Breast Cancer Tissues in Comparison with Paired Normal Marginal Tissues.
Background: Breast malignancy is a major public health problem and one of the prevalent cancers, which leads to death in women all around the world. Long non-coding RNAs (lncRNAs) are widely studied in the pathogenesis of malignancies. Previous studies have confirmed the correlation of lncRNAs and t...
| Published in: | Middle East Journal of Cancer Vol. 17; no. 2; pp. 97 - 106 |
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| Main Authors: | , , , , , , , |
| Format: | research tables/charts Journal Article |
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Middle East Journal of Cancer
Apr2026
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=194806747&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 194806747 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20086709 B7LE jtl: Middle East Journal of Cancer issn: 20086709 maglogo: N pubinfo: dt: Apr2026 vid: 17 iid: 2 pid: 67247 pub: Middle East Journal of Cancer artinfo: ui: 194806747 194806747 194806747 10.30476/mejc.2025.105196.2229 194806747 ppf: 97 ppct: 9 formats: fmt: @attributes: type: P tig: atl: Evaluation of the LncRNA TMPO AS-1 Expression Level in Breast Cancer Tissues in Comparison with Paired Normal Marginal Tissues. aug: au: Niknam, Saman Zarredar, Habib Asvadi, Tooraj Asadi, Milad Zafari, Venus Maleki, Raheleh Aligholipour Shanehbandi, Dariush Hashemzadeh, Shahryar affil: Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran sug: subj: Breast Neoplasms Physiopathology Genes RNA Analysis Human Female Middle Age Case Control Studies Funding Source Iran Comparative Studies Academic Medical Centers Iran Neoplasm Staging Reverse Transcriptase Polymerase Chain Reaction Data Analysis Software Descriptive Statistics Paired T-Tests Unpaired T-Tests ROC Curve Bioinformatics Gene Expression Chemotherapy, Cancer Tumor Markers, Biological Proteins Metabolism Breast Neoplasms Prognosis Middle Aged: 45-64 years Female ab: Background: Breast malignancy is a major public health problem and one of the prevalent cancers, which leads to death in women all around the world. Long non-coding RNAs (lncRNAs) are widely studied in the pathogenesis of malignancies. Previous studies have confirmed the correlation of lncRNAs and the cellular development and growth of breast malignancy. The present study aimed to evaluate the expression level of long non-coding RNATMPO antisense RNA 1 (LncRNA TMPO AS-1) in breast cancer tissues and compare it with paired normal marginal tissues. Method: 50 breast cancer patients were included in this case-control study and tumor tissues and healthy marginal tissues were collected during surgery. Total RNAs were extracted from the normal and cancerous tissues, and after quality control cDNA was synthesized. The TMPO AS-1 expression level was determined by quantitative polymerase chain reaction (qPCR). The gene expression level and their relationships with the patient's clinicopathological characteristics were analyzed using appropriate statistical tests. Results: TMPO Antisense RNA 1(TMPO-AS1) is significantly high expressed in breast cancer tissues compared with normal peripheral tissues (P-value <0.0001, fold change: 1.16). Among the 40 patients exposed to chemotherapy drugs 5-Fluorouracil (5FU) and doxorubicin, the TMPO-AS1 expression level was significantly decreased as compared with the 10 patients unexposed to chemotherapy (P < 0.0001, fold change: 0.11). Lymph node metastatic patients (29 positive patients, 21 negative patients) showed high TMPO-AS1 expression level than negative lymph nodes metastasis patients (P = 0.0012, fold change = 1.161). Conclusion: We demonstrated that TMPO-AS1 is highly expressed and its up-regulation correlates with the pathogenesis and tumorigenesis of breast cancer. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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