Comparison of Non-invasive Diagnostic Methods for Helicobacter Pylori Infection Before and After Treatment.

Introduction: Helicobacter pylori (H. pylori) infection is a major contributor to peptic ulcer disease and other gastrointestinal disorders. The present study aimed to investigate the effect of treatment on diagnostic outcomes by comparing two non-invasive methods--the stool antigen test and the ser...

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Publicado en:Middle East Journal of Digestive Diseases Vol. 18; no. 1; pp. 18 - 25
Autores principales: Shamsdin, Seyedeh Azra, Faramarzi, Hossein, Khazraei, Hajar, Zare, Fateme, Kamyab, Amirhossein
Formato: research tables/charts Journal Article
Publicado: Iranian Association of Gastroenterology & Hepatology Jan2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jan2026
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      pub: Iranian Association of Gastroenterology & Hepatology
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        atl: Comparison of Non-invasive Diagnostic Methods for Helicobacter Pylori Infection Before and After Treatment.
      aug:
        au:
          Shamsdin, Seyedeh Azra
          Faramarzi, Hossein
          Khazraei, Hajar
          Zare, Fateme
          Kamyab, Amirhossein
        affil: Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
      sug:
        subj:
          Noninvasive Procedures Methods
          Helicobacter Infections Diagnosis
          Immunoglobulin G4-Related Disease
          Feces Analysis
          Treatment Outcomes
          Human
          Nonexperimental Studies
          Iran
          Dyspepsia
          Immunoglobulins
          Sensitivity and Specificity
          Male
          Female
          Clarithromycin
          Amoxicillin
          Pantoprazole Sodium
          Enzyme-Linked Immunosorbent Assay
          Data Analysis Software
          Endoscopy, Gastrointestinal
          Patient Compliance
          Funding Source
          Male
          Female
      ab: Introduction: Helicobacter pylori (H. pylori) infection is a major contributor to peptic ulcer disease and other gastrointestinal disorders. The present study aimed to investigate the effect of treatment on diagnostic outcomes by comparing two non-invasive methods--the stool antigen test and the serological IgG antibody test, before and after therapy. This approach was designed to evaluate not only the diagnostic efficacy of these tools but also the changes in antibody responses following eradication treatment. Methods: This observational study was conducted from 2021 to 2022 at Shahid Motahari Clinic, affiliated with Shiraz University of Medical Sciences, Shiraz, Iran. A total of 108 patients with dyspepsia and confirmed H. pylori infection were enrolled. Diagnostic tests included stool antigen, anti-H. pylori IgG serology, and the urease breath test (UBT). Patients received standard triple therapy, with stool antigen and IgG levels measured before and one month after treatment to assess diagnostic accuracy and treatment outcomes. Results: Among 108 patients, 74% had peptic ulcer disease. The UBT showed the highest diagnostic accuracy (100% sensitivity, 94.6% specificity). Anti-H. pylori IgG serology had high sensitivity (100%) but lower specificity (66.2%). The stool antigen test demonstrated moderate accuracy. After treatment, stool antigen levels significantly decreased (from 4.2 ± 0.64 to 2.9 ± 0.75, P = 0.03), and IgG titers declined significantly (from 54.26 ± 3.33 to 46.51 ± 3.22, P < 0.0001). Conclusion: Our findings underscore UBT as the most accurate method and highlight the value of stool antigen testing for follow- up. Monitoring IgG titer changes may support treatment assessment when used alongside other tools, though its low specificity limits stand-alone use.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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