Evaluation of the Effect of Astragalus membranaceus Saponins Administration on Knee Function and Cartilage Biomarkers in Healthy Subjects with Knee Discomfort.

Objective: This study aimed to evaluate the effects of 12 weeks of Astragalus membranaceus saponins (AMS) supplementation on functional performance, knee joint mobility, self-reported outcomes, and biomarkers of inflammation and cartilage in healthy subjects with knee discomfort. Methods: A randomiz...

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Published in:Nutrients Vol. 18; no. 12; pp. 1842 - 1856
Main Authors: Zhuang, Shu Ru, Leong, Pui-Ying, Chiang, Hsin-Pei, Shen, You-Cheng
Format: research tables/charts randomized controlled trial Journal Article
Published: MDPI Jun2026
Online Access:View this record in EBSCOhost
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      dt: Jun2026
      vid: 18
      iid: 12
      pid: 97109
      pub: MDPI
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        10.3390/nu18121842
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        atl: Evaluation of the Effect of Astragalus membranaceus Saponins Administration on Knee Function and Cartilage Biomarkers in Healthy Subjects with Knee Discomfort.
      aug:
        au:
          Zhuang, Shu Ru
          Leong, Pui-Ying
          Chiang, Hsin-Pei
          Shen, You-Cheng
        affil: Department of Nutrition, Chung Shan Medical University, Taichung 402, Taiwan
      sug:
        subj:
          Astragalus Therapeutic Use
          Glycosides Therapeutic Use
          Plant Extracts Therapeutic Use
          Dietary Supplementation
          Knee Joint Physiology
          Functional Assessment
          Physical Mobility
          Patient-Reported Outcomes
          Biological Markers Blood
          Inflammation
          Cartilage, Articular Metabolism
          Knee Joint Pathology
          Treatment Outcomes
          Human
          Funding Source
          Randomized Controlled Trials
          Random Assignment
          Double-Blind Studies
          Aged
          Placebos
          Plant Extracts Administration and Dosage
          Capsules
          Step Evaluation
          Range of Motion Evaluation
          Clinical Assessment Tools
          Self Report
          Goniometry
          Flexion
          Extension
          Interleukins Blood
          Chemokines Blood
          Descriptive Statistics
          Recovery
          Exploratory Research
          Biological Markers Drug Effects
          Knee Pain Drug Therapy
          Visual Analog Scaling
          Adult
          Middle Age
          Male
          Female
          Unpaired T-Tests
          Paired T-Tests
          Wilcoxon Signed Rank Test
          Data Analysis Software
          Aged: 65+ years
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Male
          Female
      ab: Objective: This study aimed to evaluate the effects of 12 weeks of Astragalus membranaceus saponins (AMS) supplementation on functional performance, knee joint mobility, self-reported outcomes, and biomarkers of inflammation and cartilage in healthy subjects with knee discomfort. Methods: A randomized, double-blind, placebo-controlled trial was conducted in healthy subjects aged 20–70 years with knee discomfort but without clinically diagnosed knee osteoarthritis. Participants were assigned to receive one capsule of AMS or a placebo once daily for 12 weeks. The pre-specified primary endpoints were the SLSD step count and knee ROM; KOOS total score was a key secondary endpoint; serum biomarkers were exploratory. The results included functional performance assessed by the Single Leg Step Down (SLSD) test, knee range of motion (ROM), and self-reported outcomes using the Knee injury and Osteoarthritis Outcome Score (KOOS). Knee ROM was measured with a goniometer and recorded as both active ROM and passive ROM for knee flexion and extension. Serum biomarkers of inflammation (IL-8, IL-1β, MIP-1α), cartilage degradation (CTX-II, COMP, MMP-13, COL2A1), and cartilage synthesis (PIINP) were evaluated at baseline and Week 12. Results: Within the AMS group, SLSD step count increased significantly by 16.83% (Δ = +12.78 steps; p < 0.05) and recovery time decreased significantly by 19.12% (Δ = −108.91 s; p < 0.05) compared with baseline, whereas the placebo group showed smaller, non-significant changes (+4.48 steps and −56.48 s, respectively); however, neither between-group difference in change scores reached statistical significance. Significant improvements in active and passive knee ROM were observed in both flexion and extension (all p < 0.05) within the AMS group, whereas the placebo group showed no significant changes. KOOSs improved significantly in all domains within the AMS group, with the largest gains observed in sport/recreation (+22.23%) and quality of life (+18.38%). In the exploratory biomarker analysis, several inflammation and cartilage-related biomarkers changed after AMS supplementation showed within-group reductions (IL-8, COMP, MMP-13) and PIINP increased. Conclusions: 12 weeks of AMS supplementation was associated with improvements in selected functional, mobility, and outcomes in generally healthy adults with self-reported knee discomfort. AMS was also associated with changes in selected circulating biomarkers related to inflammation and cartilage metabolism. These findings should be interpreted as a preliminary, safe, complementary strategy to support joint health in healthy subjects with knee discomfort.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        randomized controlled trial
        Journal Article
      ougenre: Article
    language: English
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