Examining intra- and inter-tester reliability of post-scan dual-energy X-ray absorptiometry regional body composition analysis in athletes.

Background/objectives: The purpose of this investigation was (i) to assess the intra- and inter-tester reliability of delineating regions of interest (ROIs) in Dual-energy X-ray Absorptiometry (DXA) for measuring regional fat mass (FM), lean soft tissue (LST), and bone mineral content (BMC) and (ii)...

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Publicado en:European Journal of Clinical Nutrition Vol. 80; no. 7; pp. 717 - 725
Autores principales: Silva, Tiago R., Gonçalves, Beatriz, Poínhos, Rui, Teixeira, Vitor Hugo, Silva, Analiza M.
Formato: diagnostic images research tables/charts Journal Article
Publicado: Springer Nature Jul2026
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Jul2026
      vid: 80
      iid: 7
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      pub: Springer Nature
      place: New York, New York
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        10.1038/s41430-026-01754-x
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        atl: Examining intra- and inter-tester reliability of post-scan dual-energy X-ray absorptiometry regional body composition analysis in athletes.
      aug:
        au:
          Silva, Tiago R.
          Gonçalves, Beatriz
          Poínhos, Rui
          Teixeira, Vitor Hugo
          Silva, Analiza M.
        affil: https://ror.org/043pwc612 Faculty of Nutrition and Food Sciences, University of Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal
      sug:
        subj:
          Absorptiometry, Photon
          Body Composition
          Athletes
          Reproducibility of Results
          Human
          Male
          Female
          Child
          Adolescence
          Adult
          Middle Age
          Adipose Tissue
          Bone Density
          Image Interpretation, Computer Assisted
          Paired T-Tests
          Wilcoxon Signed Rank Test
          One-Way Analysis of Variance
          Intraclass Correlation Coefficient
          Pearson's Correlation Coefficient
          Data Analysis Software
          Descriptive Statistics
          Child: 6-12 years
          Adolescent: 13-18 years
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Male
          Female
      ab: Background/objectives: The purpose of this investigation was (i) to assess the intra- and inter-tester reliability of delineating regions of interest (ROIs) in Dual-energy X-ray Absorptiometry (DXA) for measuring regional fat mass (FM), lean soft tissue (LST), and bone mineral content (BMC) and (ii) to investigate the prevalence of inconsistencies between automatically defined ROIs and manufacturer guidelines. A secondary aim was to assess whether discrepancies in the delineation of ROIs by different testers could bias regional body composition changes over time. Methods: DXA measurements were performed with a total body scan in 86 athletes (64.0% females) from 12 sports. Two independent testers conducted post-scan analysis following the manufacturer's guidelines. Intra- and inter-tester reliability was evaluated using the intra-class correlation coefficient (ICC), mean differences, paired samples t-tests and Bland-Altman plots. Results: Intra- and inter-tester reliability was nearly perfect (ICC: 0.998-1.000) across all regions and components (FM, LST, and BMC). Mean differences were consistently <1% for all regions and components, except for left arm FM (1.4%; SD = 4.6%) in inter-tester reliability. Inconsistencies between automatically defined ROIs and manufacturer guidelines were frequent, with chest line corrections (which separate the arm from the trunk) required in 91.9% of scans. Inter-tester ICC for regional changes (n = 52) ranged from 0.668 to 0.981, with wider limits of agreement than cross-sectional estimates. Conclusion: DXA post-scan regional analysis can be performed with very high reliability and low measurement error, but manual review is essential. When monitoring changes over time, baseline and follow-up scans should be analyzed by the same evaluator.
      pubtype: Academic Journal
      doctype:
        diagnostic images
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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