Fluorescent organelle markers in Cryptococcus neoformans : a versatile toolkit for live-cell subcellular localization.
Understanding how intracellular organelles are organized and dynamically remodeled is essential for elucidating fungal growth, differentiation, and pathogenicity. The human fungal pathogen Cryptococcus neoformans exhibits remarkable morphological diversity, yet the contribution of intracellular orga...
| Publicado en: | Genetics Vol. 233; no. 3; pp. 1 - 16 |
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| Autores principales: | , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Jul2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195476933&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195476933 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Jul2026 vid: 233 iid: 3 pid: 622 pub: Oxford University Press / USA artinfo: ui: 195476933 195476933 195476933 10.1093/genetics/iyag110 195476933 ppf: 1 ppct: 15 formats: tig: atl: Fluorescent organelle markers in Cryptococcus neoformans : a versatile toolkit for live-cell subcellular localization. aug: au: Choi, Yeseul Bahn, Yong-Sun Heitman, Joseph affil: Department of Molecular Genetics and Microbiology, Duke University Medical Center, Duke University, Durham, NC 27710, United States sug: subj: Cryptococcus Analysis Cell Physiology Fluorescent Dyes Organelles Biological Markers Luminescent Measurements Human Funding Source Male Female Adult Middle Age Endoplasmic Reticulum Cytoplasm Cell Differentiation Plasmids Cell Nucleus Mitochondria Autophagy Cell Membrane Cell Division Reverse Transcriptase Polymerase Chain Reaction Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-Associated Proteins Electroporation Post Hoc Analysis Microscopy Staining and Labeling One-Way Analysis of Variance In Vitro Studies Descriptive Statistics Adult: 19-44 years Middle Aged: 45-64 years Male Female ab: Understanding how intracellular organelles are organized and dynamically remodeled is essential for elucidating fungal growth, differentiation, and pathogenicity. The human fungal pathogen Cryptococcus neoformans exhibits remarkable morphological diversity, yet the contribution of intracellular organelles to adaptation across environmental and host-relevant conditions have not been systematically examined. Here, we establish a comprehensive toolkit of fluorescent organelle marker plasmids and strains expressing mCherry or GFP fusions, enabling high-resolution live-cell imaging of 10 major subcellular compartments: the nucleolus, endoplasmic reticulum (ER), Golgi apparatus, mitochondria, peroxisome, endosome, autophagosome, vacuole, the plasma membrane, and processing bodies (P-bodies). With this platform, we analyzed organelle organization under host-relevant conditions, including elevated temperature, 5% CO2, and capsule- and melanin-inducing media, as well as throughout sexual development from zygote formation to basidiospore production. We further show that the toolkit supports colocalization analyses via diploid formation or dual-labeling strategies. Collectively, our results reveal condition- and stage-specific remodeling of organelle architecture during vegetative growth and mating. This imaging platform provides a robust framework for investigating how subcellular organization supports fungal adaptation, development, and virulence, and for inferring the functions of uncharacterized genes from their spatial localization. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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