Juglone Attenuates CCl₄‐Induced Hepatic Injury in Mice With Associated Modulation of Oxidative Stress and Inflammasome‐Related mRNA Expression.
Carbon tetrachloride–induced liver injury is a well‐established model of toxic hepatitis, characterized by marked oxidative and inflammatory damage. It remains a relevant experimental system because broadly effective therapeutic options are still limited. Juglone, a natural naphthoquinone derived fr...
| Published in: | BioMed Research International Vol. 2026; pp. 1 - 13 |
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| Main Authors: | , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
7/21/2026
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195519890&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195519890 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 7/21/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 195519890 195519890 195519890 10.1155/bmri/8905392 195519890 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Juglone Attenuates CCl₄‐Induced Hepatic Injury in Mice With Associated Modulation of Oxidative Stress and Inflammasome‐Related mRNA Expression. aug: au: Sohail, Aamir Sufyan, Raza Asim, Muhammad Khan, Mehroz Altaf, Aleena Haq, Muhammad Ehsan Ul Arshad, Maimoona Khalil, Atif Ali Khan Ullah, Imran Banerjee, Baisakhi affil: Department of Biochemistry,, Faculty of Biological Sciences,, Quaid-i-Azam University,, Islamabad, Pakistan, qau.edu.pk sug: subj: Hydrocarbons, Chlorinated Liver Diseases Chemically Induced Liver Diseases Drug Therapy Oxidative Stress Drug Effects Inflammation Mediators Drug Effects RNA, Messenger Drug Effects Gene Expression Inflammation Prevention and Control Quinones Analysis Plants, Medicinal Plant Extracts Analysis Animal Studies Mice Biological Products Models, Biological Experimental Studies Staining and Labeling Reverse Transcriptase Polymerase Chain Reaction Liver Injuries Apoptosis Endoplasmic Reticulum Stress Autophagy Weight Loss Hyperlipidemia Transcription Factors Funding Source Laboratories Pakistan Biochemistry Biological Assay Molecular Biology Data Analysis Software One-Way Analysis of Variance Post Hoc Analysis ab: Carbon tetrachloride–induced liver injury is a well‐established model of toxic hepatitis, characterized by marked oxidative and inflammatory damage. It remains a relevant experimental system because broadly effective therapeutic options are still limited. Juglone, a natural naphthoquinone derived from Reynoutria japonica, has demonstrated antioxidant and anti‐inflammatory potential. However, mouse evidence combining standard liver injury measures with stress‐ and cell‐death gene profiling remains limited. Building on juglone studies in injury and fibrosis, we tested juglone in a CCl4 model. Male C57BL/6 mice were assigned to normal, vehicle, CCl₄, silymarin (200 mg/kg), and juglone (8 mg/kg) groups, and CCl₄ (in corn oil) was administered intraperitoneally. Endpoints included body weight, serum liver enzymes and lipid profile, H&E histology, and RT‐qPCR panels for inflammatory, pyroptosis‐associated, ER stress and autophagy‐linked, apoptotic, oxidative‐stress, and profibrotic transcripts. CCl₄ exposure was associated with weight loss, enzyme elevation, dyslipidemia, and architectural disruption, whereas juglone treatment was associated with improved biochemical indices and histology alongside coordinated downregulation of inflammatory/pyroptosis‐related transcripts (including Il-1β, Il-6, Nlrp3, and Gsdmd) and moderation of ER stress/autophagy and apoptosis‐linked transcripts, with downregulation of profibrotic transcripts (Acta2, Mmp2, and Tgfβ1). In this mouse CCl₄ model, juglone treatment was associated with reduced serum and tissue injury signatures together with changes in stress and inflammation‐linked transcriptional programs. Because the molecular findings are primarily based on RT‐qPCR, these results are best interpreted as gene‐level associations compatible with modulation of oxidative stress, inflammasome‐ and pyroptosis‐related mRNA expression, ER stress responses, apoptosis‐linked transcripts, and remodeling‐associated transcripts, rather than as confirmed pathway inhibition or established antifibrotic activity. These data support juglone as a hepatoprotective candidate and identify protein‐level and functional validation as important next steps. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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