STAT6 Immunohistochemistry.
Context.--Eosinophilic esophagitis (EoE) is a chronic, immune-mediated condition with overlapping histologic features with gastroesophageal reflux disease and other esophagitis subtypes. Current diagnostic criteria based on eosinophil density and basal zone hyperplasia lack specificity, necessitatin...
| Publicado en: | Archives of Pathology & Laboratory Medicine Vol. 150; no. 7; pp. 559 - 564 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
College of American Pathologists
Jul2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195527028&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195527028 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00039985 1FS jtl: Archives of Pathology & Laboratory Medicine issn: 00039985 maglogo: N pubinfo: dt: Jul2026 vid: 150 iid: 7 pid: 2550 pub: College of American Pathologists place: Northfield, Illinois artinfo: ui: 195527028 195527028 195527028 10.5858/arpa.2025-0473-OA 195527028 ppf: 559 ppct: 5 formats: fmt: @attributes: type: P tig: atl: STAT6 Immunohistochemistry. aug: au: Mayer, Chen Dahan, Osher Levartovsky, Asaf Barshack, Iris Goldman, Adam Schvimer, Michael affil: Institute of Pathology, Sheba Medical Center, Ramat Gan, Israel sug: subj: Eosinophilic Esophagitis Diagnosis Immunohistochemistry STAT Proteins Esophagus Pathology Biopsy Hyperplasia Eosinophils Analysis Human Male Female Descriptive Statistics Multiple Linear Regression Spearman's Rank Correlation Coefficient Retrospective Design Record Review Israel Mann-Whitney U Test Sensitivity and Specificity Staining and Labeling Data Analysis Software Predictive Value of Tests ROC Curve Infant Child, Preschool Child Adolescence Adult Middle Age Aged Aged, 80 and Over Infant: 1-23 months Child, Preschool: 2-5 years Child: 6-12 years Adolescent: 13-18 years Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Aged, 80 & over Male Female ab: Context.--Eosinophilic esophagitis (EoE) is a chronic, immune-mediated condition with overlapping histologic features with gastroesophageal reflux disease and other esophagitis subtypes. Current diagnostic criteria based on eosinophil density and basal zone hyperplasia lack specificity, necessitating more reliable tissue biomarkers. Objective.--To evaluate the diagnostic utility of signal transducer and activator of transcription 6 (STAT6) immu-nohistochemistry (IHC) in distinguishing EoE from histologic mimics in esophageal biopsies. Design.--STAT6 IHC was performed on 208 esophageal biopsy specimens, including cases of EoE, treated EoE, reflux esophagitis, other esophagitis, and normal mucosa. A blinded gastrointestinal pathologist scored basal epithelial nuclear staining. Diagnostic performance was assessed using predefined and optimized thresholds. Correlations with eosino-phil density and basal zone hyperplasia were analyzed using Spearman correlation and multivariable linear regression. Results.--Among 151 well-characterized cases, a 50% nuclear staining threshold yielded 95.6% sensitivity and 97.2% specificity for EoE. An optimized 32.5% threshold achieved 100% sensitivity and 95.3% specificity. STAT6 expression strongly correlated with eosinophil density (p = 0.80) and basal zone hyperplasia (p = 0.69), but was not entirely explained by either, indicating STAT6 reflects distinct T helper type 2-mediated epithelial activity. Residual staining was observed in some treated EoE cases despite histologic remission. Conclusions.--STAT6 IHC is a robust, specific bio-marker for EoE that offers high diagnostic accuracy and reflects underlying T helper type 2-driven epithelial signaling. Its application may improve diagnostic precision and support future efforts in disease monitoring and therapeutic response assessment. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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