Superior In Vitro Osteo-Supportive Properties of Trabecular Titanium vs. Chromium–Cobalt Scaffolds.
Background: Degenerative joint diseases are a major cause of disability and drive the increasing demand for joint arthroplasty. Long-term prosthesis success depends on rapid and stable bone–implant integration, which is influenced by the osteo-inductive and osteo-conductive properties of implant mat...
| Publicado en: | Prosthesis (2673-1592) Vol. 8; no. 7; pp. 70 - 92 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
MDPI
Jul2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195806954&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195806954 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26731592 MVQF jtl: Prosthesis (2673-1592) issn: 26731592 maglogo: N pubinfo: dt: Jul2026 vid: 8 iid: 7 pid: 97109 pub: MDPI artinfo: ui: 195806954 195806954 195806954 10.3390/prosthesis8070070 195806954 ppf: 70 ppct: 22 formats: tig: atl: Superior In Vitro Osteo-Supportive Properties of Trabecular Titanium vs. Chromium–Cobalt Scaffolds. aug: au: Nebuloni, Andrea Massimiliano Lauro, Roberta Taiana, Michela Maria Sorano, Gaetano Costa, Piero Ragni, Enrico de Girolamo, Laura affil: Laboratorio di Biotecnologie Applicate all'Ortopedia, IRCCS Ospedale Galeazzi–Sant'Ambrogio, Via Crisitina Belgioioso 173, 20157 Milano, Italy sug: subj: Titanium Pharmacodynamics Chromium Compounds Pharmacodynamics Biocompatible Materials Osteogenesis Tissue Scaffolds Human In Vitro Studies Comparative Studies Titanium Analysis Chromium Compounds Analysis Mesenchymal Stem Cells Cell Differentiation Bone Regeneration Cell Proliferation Cell Viability Alkaline Phosphatase Calcium Gene Expression Flow Cytometry Reverse Transcriptase Polymerase Chain Reaction Multivariate Analysis One-Way Analysis of Variance Post Hoc Analysis Kruskal-Wallis Test Paired T-Tests Mann-Whitney U Test Data Analysis Software Descriptive Statistics Funding Source ab: Background: Degenerative joint diseases are a major cause of disability and drive the increasing demand for joint arthroplasty. Long-term prosthesis success depends on rapid and stable bone–implant integration, which is influenced by the osteo-inductive and osteo-conductive properties of implant materials. Chromium–cobalt (CrCo) and titanium (Ti) alloys are widely used in reconstructive orthopedics, but direct comparative data on their biological performance, particularly for trabecular titanium (T-Ti), remain limited. This study aimed to directly compare the biocompatibility and osteogenic potential of CrCo and T-Ti using human mesenchymal stromal cells (MSCs). Methods: Human MSCs were characterized by immunophenotyping and cultured on CrCo and T-Ti scaffolds under control and osteogenic conditions for up to 28 days. Cell adhesion and morphology were assessed by scanning electron microscopy. Proliferation and viability were quantified, and osteogenic differentiation was evaluated using alkaline phosphatase activity, calcium deposition assays, and gene expression profiling of osteogenic markers. Results: Both materials supported MSC adhesion and proliferation, confirming cytocompatibility. Under control conditions, T-Ti significantly increased alkaline phosphatase activity and osteogenic gene expression. Under osteogenic stimulation, T-Ti accelerated differentiation and mineralized matrix deposition. CrCo exhibited limited stimulation of the osteogenic-supportive microenvironment and delayed differentiation responses. Conclusions: Trabecular titanium, in terms of morphology and topology, provides a biologically active scaffold that both induces and conducts osteogenic differentiation of human MSCs, whereas CrCo acts primarily as a mechanically optimized but biologically passive material. These findings support the use of trabecular titanium at bone-contact interfaces in joint prostheses to enhance osteointegration and potentially improve long-term implant stability. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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