High Histological Entropy Is Correlated with Poor Overall Survival and Death Within the First 2 Years in Diffuse Large B-Cell Lymphoma.
Simple Summary: Diffuse large B-cell lymphoma (DLBCL) is an aggressive lymphoma and one of the most common hematological neoplasms. To date, it is not easy to identify DLBCL patients with aggressive clinical evolution and death within the first 2 years after diagnosis, only by using hematoxylin and...
| Publicado en: | Cancers Vol. 18; no. 14; pp. 2279 - 2304 |
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| Autores principales: | , , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
MDPI
Jul2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195807569&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195807569 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20726694 B74B jtl: Cancers issn: 20726694 maglogo: N pubinfo: dt: Jul2026 vid: 18 iid: 14 pid: 97109 pub: MDPI artinfo: ui: 195807569 195807569 195807569 10.3390/cancers18142279 195807569 ppf: 2279 ppct: 25 formats: tig: atl: High Histological Entropy Is Correlated with Poor Overall Survival and Death Within the First 2 Years in Diffuse Large B-Cell Lymphoma. aug: au: Carreras, Joaquim Kikuti, Yara Yukie Nagase, Shunsuke Roncador, Giovanna Ikoma, Haruka Ito, Atsushi Orita, Makoto Tomita, Sakura Tanigaki, Yuki Ueno, Akihisa Kondo, Yusuke Nakamura, Naoya Masugi, Yohei affil: Department of Pathology, School of Medicine, Tokai University, 143 Shimokasuya, Isehara 259-1193, Japan sug: subj: Lymphoma, B-Cell Prognosis Lymphoma, B-Cell Mortality Staining and Labeling Image Processing, Computer Assisted Human Male Female Funding Source Japan Academic Medical Centers Retrospective Design Medical Records Record Review Immunohistochemistry Gene Expression Profiling Mann-Whitney U Test Kruskal-Wallis Test Chi Square Test Kaplan-Meier Estimator Survival Analysis Descriptive Statistics Data Analysis Software Male Female ab: Simple Summary: Diffuse large B-cell lymphoma (DLBCL) is an aggressive lymphoma and one of the most common hematological neoplasms. To date, it is not easy to identify DLBCL patients with aggressive clinical evolution and death within the first 2 years after diagnosis, only by using hematoxylin and eosin (H&E) histological images. This study used the entropy function to analyze H&E images of DLBCL to measure randomness, to characterize the texture of an input image, and to measure tissue complexity. Image processing and computer vision analysis were performed on a series of 114 diagnostic DLBCL cases and 44 reactive lymphoid tissues stained with H&E. In comparison with reactive lymphoid tissue, DLBCL was characterized by lower entropy. Within the diagnostic category of DLBCL, higher entropy correlated with poor overall survival and death event within the first two years. In conclusion, the histological evaluation of entropy is useful for both the differential diagnosis of reactive lymphoid tissue and DLBCL and can be used as a predictive factor for DLBCL prognosis. Background/Objectives: Diffuse large B-cell lymphoma (DLBCL) is an aggressive lymphoma and one of the most common hematological neoplasia. Entropy is a statistical measure of randomness that characterizes the texture of an input image and measures tissue complexity. Methods: Image processing and computer vision analysis were performed on a series of 114 diagnostic DLBCL cases and 44 reactive lymphoid tissues stained with hematoxylin and eosin (H&E). Histological entropy was measured to differentiate between reactive lymphoid tissue and DLBCL and predict clinical evolution. At protein level, immunohistochemistry analyzed Ki67, LMO2, MYC, MDM2, CDK6, E2F1, BCL2, CASP8, MYOB, TP53, cPARP, cCASP3, ISY1, TNFAIP8, CSF1R, CD163, PD-L1 and IL-10 markers. Gene expression analysis using the NanoString nCounter PanCancer Immune Profiling Panel was performed in 29 cases. Results: In comparison with reactive lymphoid tissue, DLBCL was characterized by lower entropy (7.32 ± 0.16 vs. 6.82 ± 0.44, respectively; p < 0.001). Within the DLBCL diagnostic category, higher entropy was associated with poor overall survival and death events within the first 2 years (hazard-risk = 2.4, p = 0.004) and lower entropy with a moderate and more favorable outcome (hazard-risk = 0.4, p = 0.004). High entropy was also correlated with ECOG performance status ≥ 2, lower protein expression of apoptosis markers of cPARP and cCASP3, and upregulation of specific immuno-oncology genes such as STAT3, BTK, CASP8, CD47, VCAM1, and MYD88. The prognostic value of entropy was independent of the international prognostic index (IPI), Epstein–Barr virus (EBER), and cell of origin (Hans). Conclusions: The histological evaluation of entropy is useful for the differential diagnosis of reactive lymphoid tissue and DLBCL and is a predictive factor of DLBCL prognosis. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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