Fecal Carriage of ESBL and Carbapenem Resistance among hospitalized patients in Tehran, Iran.
Background & Objective: Enterobacteriaceae, a family of Gram-negative bacteria in the normal gut flora, are common human pathogens. The rising fecal carriage of carbapenemase-producing (CPE) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) poses significant resistance to beta...
| Publicado en: | Journal of Advances in Medical & Biomedical Research Vol. 34; no. 2; pp. 156 - 166 |
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| Autores principales: | , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Zanjan University of Medical Sciences & Health Services
Mar/Apr2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=195814692&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 195814692 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 26766264 N5MJ jtl: Journal of Advances in Medical & Biomedical Research issn: 26766264 maglogo: N pubinfo: dt: Mar/Apr2026 vid: 34 iid: 2 pid: 65276 pub: Zanjan University of Medical Sciences & Health Services artinfo: ui: 195814692 195814692 195814692 10.30699/jambr.34.2.156 195814692 ppf: 156 ppct: 10 formats: fmt: @attributes: type: P tig: atl: Fecal Carriage of ESBL and Carbapenem Resistance among hospitalized patients in Tehran, Iran. aug: au: Omidi, Amir Hossein Seydmohammadi, Reyhaneh Mohammadi, Mohammad Reza Latifian, Mina Nikbin, Vajihe Sadat Atabi, Fereshteh Doust, Reza Hosseini Shahcheraghi, Fereshteh affil: Department of Epidemiology and Biostatics, Research Centre for Emerging and Reemerging Infectious Diseases, Pasteur Institute of Iran, Tehran, Iran. sug: subj: Enterobacteriaceae Infections Epidemiology Enterobacteriaceae Infections Familial and Genetic Feces Microbiology Carbapenem-Resistant Enterobacteriaceae Antibiotics, Lactam Drug Resistance, Multiple Genetics, Microbial Hospitalization Human Iran Inpatients Prevalence Intensive Care Units Microbial Culture and Sensitivity Tests Polymerase Chain Reaction Klebsiella Infections Familial and Genetic Escherichia Coli Infections Familial and Genetic Cross Sectional Studies Data Analysis Software Male Female Descriptive Statistics Male Female ab: Background & Objective: Enterobacteriaceae, a family of Gram-negative bacteria in the normal gut flora, are common human pathogens. The rising fecal carriage of carbapenemase-producing (CPE) and extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) poses significant resistance to beta-lactam antibiotics. Accordingly, this study investigated the prevalence and molecular characterization of beta-lactamase genes (blaKPC, blaIMP, blaVIM, blaNDM, blaSHV, blaTEM, blaOXA, and blaCTX-M) in Enterobacteriaceae isolates from hospitalized patients in Shahriar, Iran. Materials & Methods: A total of 85 stool samples were collected from hospitalized patients in the ICU and general wards of Imam Sajjad Hospital, Shahriar. Bacterial isolates were identified using standard microbiological methods for identification of Enterobacteriaceae. Antibiotic susceptibility profile of isolates was analysed by using the disc diffusion, and Minimum inhibitory concentrations (MICs) method based on the Clinical and Laboratory Standards Institute (CLSI) 2023 guidelines. Phenotypic detection of ESBL and such as metallo-beta-lactamase (MBL) resistance, was performed using combined disc tests with specific inhibitors. PCR amplification with specific primer was used for detection beta-lactamase genes including blaKPC, blaIMP, blaVIM, blaNDM, blaSHV, blaTEM, blaOXA and blaCTX-M. Results: From 85 samples, 19 isolates of Klebsiella pneumoniae (20.43%) and 66 isolates of Escherichia coli (79.57%) were identified. Among these, 31 isolates exhibited ESBL production, and 10 were identified as MBL [-producing isolates]. Among the 31 carbapenem-resistant or intermediate isolates, all carried the blaCTXM gene. Additionally, 2 isolates carried the blaNDM-1 gene, and another 2 harbored the blaIMP gene. However, the blaKPC and blaVIM genes were not detected in any of the isolates. Conclusion: The blaCTX-M gene is one of the most common resistant genes in Iran. According to studies, the prevalence of antibiotic resistance in Iran is rising dramatically, which reduces the choice of antibiotics to treat severe infections in the future pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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