rs2357322 and rs17726078 Contribute to Breast Cancer Risk by Regulating DLX2 Expression.
Background and Aims: Breast cancer is the most abundant cancer type in female. Genome‐wide association study suggests that rs2016394, one SNP at the intron of DLX2 divergent transcript (DLX2-DT), is significantly associated with this disease. Through 1000 genomes project data analysis, it is observe...
| Publicado en: | Breast Journal Vol. 2026; pp. 1 - 9 |
|---|---|
| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
8/14/2026
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=196220452&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 196220452 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1075122X ET6 jtl: Breast Journal issn: 1075122X maglogo: Y pubinfo: dt: 8/14/2026 vid: 2026 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 196220452 196220452 196220452 10.1155/tbj/3096873 196220452 ppf: 1 ppct: 8 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: rs2357322 and rs17726078 Contribute to Breast Cancer Risk by Regulating DLX2 Expression. aug: au: Zhang, Xin-Xin Guo, Hao Li, Hai-Yan Zhou, Xi-Ting Song, Hong-Li Xu, Jiang-Wei Fu, Yu-Hang Tian, Ru-Hui Jiang, Ru Liu, Chun-Chun Sun, Chang Sharma, Karuna affil: College of Life Sciences, , Shaanxi Normal University, , Xi'an Shaanxi, 710119, , China, snnu.edu.cn sug: subj: Gene Expression Breast Neoplasms Familial and Genetic Polymorphism, Single Nucleotide Breast Neoplasms Risk Factors Genomics Gene Editing Chromosome Mapping In Vitro Studies Genetic Techniques Descriptive Statistics Data Analysis Software T-Tests Cell Culture Techniques Reverse Transcriptase Polymerase Chain Reaction Mutation Funding Source Genome Wide Association Study Sequence Analysis Alleles Transcription Factors Oncogenes RNA, Messenger Cell Proliferation Wound Healing ab: Background and Aims: Breast cancer is the most abundant cancer type in female. Genome‐wide association study suggests that rs2016394, one SNP at the intron of DLX2 divergent transcript (DLX2-DT), is significantly associated with this disease. Through 1000 genomes project data analysis, it is observed that another three SNPs, rs743605, rs2357322, and rs17726078, show strong linkage disequilibrium with rs2016394. However, the functional SNP(s) and mechanism are still unknown. Methods: Functional genomics effort was performed for this locus. Results: Through luciferase assay, it is disclosed that rs743605 and rs2016394 are not with the ability to alter gene expression. In contrast, rs2357322 and rs17726078 alleles present significantly different luciferase expression, thus suggesting that these two SNPs are functional mutations. Chromosome conformation capture indicates that DLX2 (distal‐less Homeobox 2) can interact with the cis‐regulatory element containing these two SNPs and should be the regulatory target. Chromatin immunoprecipitation suggests that transcription factor MYC (MYC proto‐oncogene, bHLH transcription factor) can bind the rs17726078 surrounding region. Knock‐out of the segment containing these two SNPs by CRISPR/Cas9 can significantly increase DLX2 mRNA and protein expression, cell proliferation, colony formation, migration, and wound healing, thus suggesting that the cis‐regulatory element is an attenuator for gene expression. Conclusion: rs2357322 and rs17726078 might influence DLX2 expression and further contribute to breast cancer risk. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|