Peripubertal Exposure to Silver Nanoparticles (AgNPs) Triggers Follicle Activation, Probably Leading to "Burnout" by Stimulating the PI3K/AKT/mTOR/AMPK Signaling Pathway.
Despite the widespread use of silver nanoparticles (AgNPs) in consumer, industrial, and biomedical products, their potential effects on female reproductive health, particularly during critical developmental periods, remain insufficiently understood. The present study was therefore designed to invest...
| Publicado en: | Physiological Research Vol. 75; no. 4; pp. 745 - 759 |
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| Autores principales: | , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Institute of Physiology, Academy of Sciences of the Czech Republic
Aug2026
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=196834428&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 196834428 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 08628408 1CVW jtl: Physiological Research issn: 08628408 maglogo: N pubinfo: dt: Aug2026 vid: 75 iid: 4 pid: 24634 pub: Institute of Physiology, Academy of Sciences of the Czech Republic artinfo: ui: 196834428 10.33549/physiolres.935737 196834428 ppf: 745 ppct: 14 formats: fmt: @attributes: type: P tig: atl: Peripubertal Exposure to Silver Nanoparticles (AgNPs) Triggers Follicle Activation, Probably Leading to "Burnout" by Stimulating the PI3K/AKT/mTOR/AMPK Signaling Pathway. aug: au: NEHDI, Saber ALDAHMASH, Waleed ALHAZMI, Abeer FERIANI, Anouar JALOULI, Maroua ALHELAISI, Awadh SHAIK, Jilani Purusottapatnam ALWASEL, Saleh HARRATH, Abdel Halim affil: Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia. sug: ab: Despite the widespread use of silver nanoparticles (AgNPs) in consumer, industrial, and biomedical products, their potential effects on female reproductive health, particularly during critical developmental periods, remain insufficiently understood. The present study was therefore designed to investigate in vivo the dose-dependent effects of AgNP exposure on ovarian function and reproductive health in pubertal female rats. Pubertal Wistar albino female rats were divided into control and two AgNP groups (0.05 and 0.50 mg/kg/day). After one month of therapy, the females in the different groups were euthanized, and ovarian samples were collected. The results indicated that administration of AgNPs significantly increases the number of primordial follicles and significantly increases the number of growing follicles. Western blot analysis revealed that aromatase (Cyp19) and proliferating cell nuclear antigen (PCNA) were upregulated in both treatment groups. Similarly, the number of mRNA transcripts associated with folliculogenesis and steroidogenesis increased significantly in the high-dose treatment group. In addition, the level of growth differentiation factor 9 (GDF9) mRNA increased significantly in a dose-dependent manner. However, the levels of anti-Müllerian hormone (Amh) mRNA were considerably higher in the low-dose group and lower in the high-dose group, demonstrating that the detailed regulation of this gene is affected by AgNP dose. Analysis of the phosphatidylinositol 3-kinase signaling pathway revealed that compared to a 0.05 mg/kg, a AgNP exposure at 0.5 mg/kg significantly enhanced the PI3K/AKT/mTOR pathway and impacted Ampk signaling in a distinct manner. These findings suggest that AgNPs increase follicular development and deplete the follicular reserve, resulting in a phenomenon known as "burnout". pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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