Diagnosis of Helicobacter pylori infection with a new non-invasive antigen-based assay. HpSA European study group.
Background: Helicobacter pylori is a common human pathogen implicated in certain gastrointestinal diseases. In the search for new non-invasive techniques to diagnose H. pylori infection, we evaluated an EIA for H. pylori antigen in stool (HpSA).Methods: In a prospective multicentre study, stool spec...
| Publicado en: | Lancet Vol. 354; no. 9172; pp. 30 - 34 |
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| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Lancet
7/3/1999
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=2005978&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 2005978 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01406736 LAN jtl: Lancet issn: 01406736 maglogo: N pubinfo: dt: 7/3/1999 vid: 354 iid: 9172 pid: 1297 pub: Lancet place: Philadelphia, Pennsylvania artinfo: ui: 2005978 2005978 NLM10406362 2005978 10.1016/S0140-6736(98)08103-3 NLM10406362 2005978 ppf: 30 ppct: 4 formats: fmt: @attributes: type: P tig: atl: Diagnosis of Helicobacter pylori infection with a new non-invasive antigen-based assay. HpSA European study group. aug: au: Vaira, Dino Malfertheiner, Peter Megraud, Francis Axon, Anthony TR Deltenre, Michel Hirschl, Alexander M. Gasbarrini, Giovanni O'Morain, Colm Garcia, Jose M Pajares Quina, Mario Tytgat, Guido NJ Vaira, D Malfertheiner, P Mégraud, F Axon, A T Deltenre, M Hirschl, A M Gasbarrini, G O'Morain, C Garcia, J M affil: Clinica Medica I, Università di Bologna, Italy sug: subj: Antigens, Bacterial Analysis Gastritis Diagnosis Helicobacter Infections Diagnosis Helicobacter Pylori Immunology Peptic Ulcer Diagnosis Prospective Studies Predictive Value of Tests Adult Helicobacter Infections Immunology Gastritis Immunology Gastroscopy Human Gastric Mucosa Pathology Peptic Ulcer Immunology Biopsy Gastritis Pathology Immunoenzyme Techniques Adolescence Aged, 80 and Over Female Helicobacter Infections Pathology Aged Male Peptic Ulcer Pathology Feces Middle Age Validation Studies Comparative Studies Evaluation Research Multicenter Studies Adult: 19-44 years Adolescent: 13-18 years Aged, 80 & over Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Background: Helicobacter pylori is a common human pathogen implicated in certain gastrointestinal diseases. In the search for new non-invasive techniques to diagnose H. pylori infection, we evaluated an EIA for H. pylori antigen in stool (HpSA).Methods: In a prospective multicentre study, stool specimens from 501 patients (276 men, 225 women; age range 17-88 years, mean 52) undergoing gastroscopy in 11 centres throughout Europe were tested with HpSA and the carbon-13-urea breath test. At endoscopy, four biopsy samples were taken for histology (haematoxylin and eosin) and H. pylori detection (giemsa in both antrum and corpus, culture and rapid urease test). Patients were defined as positive for H. pylori if histology (antrum, corpus, or both) and urease test were positive, or if culture was positive. Patients classified as having H. pylori infection received an eradication regimen; 107 were reassessed 4 weeks after therapy.Findings: Of 272 patients with H. pylori infection by the predefined criteria, 256 were positive by HpSA (sensitivity 94.1% [95% CI 90.6-96.6]). Of 219 patients without infection, 201 were negative by HpSA (specificity 91.8% [87.3-95.1]).Interpretation: The stool assay was a reliable and easy-to-use tool for diagnosis of H. pylori infection. The test was accurate even shortly after treatment. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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