Isolation and characterization of Bordetella bronchiseptica strains from canine origin.

Bordetella bronchiseptica strains were isolated, identified and characterized from canine origin. With this aim, dogs with semiology of respiratory clinical signs or clinically healthy were sampled with hyssop impregnated of brain-heart infusion medium. Swabs were cultured on Mac Conkey agar plates...

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Publicado en:Veterinaria México Vol. 37; no. 3; pp. 313 - 326
Autores principales: González, Graciela Molina, Rosales, María Eugenia, Morales, Gabriela Bárcenas, Crespo, Juan Antonio Montaraz
Formato: Artículo
Publicado: Facultad de Medicina Veterinaria y Zootecnia UNAM jul-sep2006
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Acceso en línea:Ver este registro en EBSCOhost
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      dt: jul-sep2006
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      pub: Facultad de Medicina Veterinaria y Zootecnia UNAM
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        atl: Isolation and characterization of Bordetella bronchiseptica strains from canine origin.
      aug:
        au:
          González, Graciela Molina
          Rosales, María Eugenia
          Morales, Gabriela Bárcenas
          Crespo, Juan Antonio Montaraz
        affil:
          Módulo de Biología Celular y Bioquímica, Facultad de Estudios Superiores-Iztacala, Los Reyes-Iztacala, Estado de México, México
          Laboratorio de Inmunología, Coordinación de Estudios de Posgrado, Facultad de Estudios Superiores-Cuautitlán, Universidad Nacional Autónoma de México, Cuautitlán-Izcalli, 54700, Estado de México, México
      su:
        Bordetella
        Respiratory diseases
        Dog diseases
        Microbial virulence
        Hemolysis & hemolysins
        Toxins
      sug:
        subj:
          Bordetella
          Respiratory diseases
          Dog diseases
          Microbial virulence
          Hemolysis & hemolysins
          Toxins
      keyword:
        BORDETELLA BRONCHISEPTICA
        RESPIRATORY DISEASE IN DOGS
      ab: Bordetella bronchiseptica strains were isolated, identified and characterized from canine origin. With this aim, dogs with semiology of respiratory clinical signs or clinically healthy were sampled with hyssop impregnated of brain-heart infusion medium. Swabs were cultured on Mac Conkey agar plates and after 48 h incubation at 37°C; the supposed colonies of B. bronchiseptica were identified by motility, Gram stain, and biochemical tests. The strains were characterized by their virulence factors such as: haemolysis, haemagglutination, production of dermonecrotic toxin and the presence of pertactin. One hundred and thirty swabs were obtained during an eight months period, and 11 strains were isolated. Eleven animals displayed tract respiratory affection and six strains were isolated. None of the strains showed a clear hemolytic activity, the hemagglutination titer varied among the different strains. All but one showed dermonecrotic activity, and only four strains expressed pertactin.
      pubtype: Academic Journal
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      src: R
    language: English
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      custom: Copyright of Veterinaria México is the property of Facultad de Medicina Veterinaria y Zootecnia UNAM and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use.
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