Oligo-Asp Tag/Zn(Il) Complex Probe as a New Pair for Labeling and Fluorescence Imaging of Proteins.
To accomplish the selective labeling of a specific protein in complicated biological systems, a peptide tag incorporated into the protein and a complementary small molecular probe are required. Although a variety of peptide tag/probe pairs have been developed as molecular tools for protein analyses,...
| Publicado en: | Journal of the American Chemical Society Vol. 128; no. 32; pp. 10452 - 10460 |
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| Autores principales: | , , , , , |
| Formato: | Artículo |
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American Chemical Society
8/16/2006
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=22270072&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 22270072 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 8/16/2006 vid: 128 iid: 32 pid: 997 pub: American Chemical Society artinfo: ui: 22270072 10.1021/ja0618604 ppf: 10452 ppct: 8 formats: tig: atl: Oligo-Asp Tag/Zn(Il) Complex Probe as a New Pair for Labeling and Fluorescence Imaging of Proteins. aug: au: Ojida, Akio Honda, Kei Shinmi, Daisuke Kiyonaka, Shigeki Mori, Yasuo Hamachi, Itaru affil: Contribution from the Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura Campus, Nishikyo-ku, Kyoto, 615-8510, Japan su: Proteins Transition metal complexes Fluorescence Imaging systems Peptides sug: subj: Proteins Transition metal complexes Fluorescence Imaging systems Peptides ab: To accomplish the selective labeling of a specific protein in complicated biological systems, a peptide tag incorporated into the protein and a complementary small molecular probe are required. Although a variety of peptide tag/probe pairs have been developed as molecular tools for protein analyses, the availability of pairs suitable for real-time imaging of proteins is still limited. We now report a new peptide tag/artificial probe pair composed of a genetically encodable oligo-aspartate sequence (D4 tag, (D4), n = 1-3) and the corresponding multinuclear Zn(ll) complexes (Zn(ll)-DpaTyrs). The strong binding affinity of the Zn(ll)-DpaTyr probes with the D4 tag was a result of the multiple coordination bonds and the multivalent effect. It was measured quantitatively by isothermal titration calorimetry. The high affinity between the tag and the probe, indispensable for the selective protein labeling, enabled the pair to be used for the labeling and fluorescence imaging of a membrane-bound receptor protein tethering a triply repeated D4 tag ((D4)) in an intact cell configuration without significantly affecting the receptor signal transduction. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2006 holdings: @attributes: islocal: N |
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