PREVALENCE OF HELICOBACTER PYLORI INFECTION AND CHARACTERISTICS OF STRAINS IN LA HABANA, CUBA.
There is a great paucity of information about Helicobacter pylori infection in the countries of the Caribbean basin. Almost no studies have been performed to determine the prevalence, antibiotic resistance or virulence factors of the bacterium. To measure the prevalence of H. pylori infection among...
| Publicado en: | Revista VacciMonitor (Vacunología y Temas Afines) Vol. 11; no. 4; pp. 1 - 2 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | Artículo |
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Vacunas Finlay S.A.
Oct2002
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=27104911&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 27104911 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 1025028X 2DU0 jtl: Revista VacciMonitor (Vacunología y Temas Afines) issn: 1025028X maglogo: N pubinfo: dt: Oct2002 vid: 11 iid: 4 pid: 30675 pub: Vacunas Finlay S.A. artinfo: ui: 27104911 ppf: 1 ppct: 1 formats: fmt: @attributes: type: P size: 12KB tig: atl: PREVALENCE OF HELICOBACTER PYLORI INFECTION AND CHARACTERISTICS OF STRAINS IN LA HABANA, CUBA. aug: au: Gutiérrez, B. Camou-Juncas, C. Santos, A. Vidal, T. Valmaña, C. González, N. Leonard, I. Martínez, R. Díaz-Canel, O. Paniagua, M. Escobar, M. P. Sierra, G. Mendez, G. L. Mégraud, Francis affil: Instituto Finlay, Ciudad de la Habana, Cuba Laboratoire de Bactériologie, Université Victor Segalen Bordeaux, Bordeaux, France Instituto de Oncología, Ciudad de La Habana, Cuba CCE, Hospital Universitario Calixto García, Ciudad de La Habana, Cuba Instituto de Gastroenterología, Ciudad de La Habana, Cuba School of Biotechnology and Biomolecular Science, The University of New South Wales, Sydney, Australia su: Helicobacter pylori infections Disease prevalence Antibiotics Drug resistance Microbial virulence Havana (Cuba) Cuba sug: subj: Havana (Cuba) Cuba Helicobacter pylori infections Disease prevalence Antibiotics Drug resistance Microbial virulence ab: There is a great paucity of information about Helicobacter pylori infection in the countries of the Caribbean basin. Almost no studies have been performed to determine the prevalence, antibiotic resistance or virulence factors of the bacterium. To measure the prevalence of H. pylori infection among patients attending endoscopy in three clinics in Havana, Cuba, to evaluate clarithromycin resistance, and to determine the cagA status of the strains obtained. Endoscopy was performed and biopsies were obtained from 117 successive patients attending the Institute of Oncology, the Institute of Gastroenterology, and the Calixto García Hospital in Havana, Cuba. Biopsies were maintained at -70°C before being cultured on 3 different media (2 selective and one non-selective) and incubated for 7 days at 37°C under a microaerobic atmosphere. The presence of H.pylori was identified by oxidase, catalase, and urease activities. DNA was extracted, and PCR was performed with primers H276/676 which amplify a 397 bp fragment of the cagA gene. Clarithromycin susceptibility was measured by the gel diffusion method. The diagnoses of patients were: 1 gastric carcinoma; 19 duodenal ulcers; 8 gastric ulcers; and 88 non-ulcer dyspepsia, including 62 gastritis, 9 hiatal hernia, 2 biliary reflux, 1 gastric polyps, and 15 no abnormality. Among the 117 biopsies tested, 83 were H. pylori positive (70.9%). The cagA status determined for 35 cases gave a positive result in 31 cases (88.5%). Only 3% of the strains were resistance to clarithromycin. The prevalence of Helicobacter pylori infection in the symptomatic population of La Habana is the same as reported for other developing countries. Most strains were cagA positive and are likely harbor the cag pathogenicity island. The low resistance to clarithromycin in the strains studied probably reflects the low degree of use of the antibiotic in this population. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista VacciMonitor (Vacunología y Temas Afines) is the property of Vacunas Finlay S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista VacciMonitor (Vacunología y Temas Afines) holder: Vacunas Finlay S.A. dt: @attributes: year: 2002 holdings: @attributes: islocal: N |
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