PEROXIDASE-LABELED STICHOLYSINS METHOD FOR LOCALIZATION OF STICHOLYSINS IN THE SEA ANEMONE STICHODACTYLA HELIANTHUS.
Sticholysins I and II are the major cytolysins present in the venom of the sea anemone Stichodactyla helianthus. These polypeptides have been isolated, purified and characterized (Lanio et al. 2001), and their immunogenic and antigenic properties studied (Pico et al. 2000). Anemones were collected i...
| Publicado en: | Revista VacciMonitor (Vacunología y Temas Afines) Vol. 11; no. 4; pp. 1 - 2 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
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Vacunas Finlay S.A.
Oct2002
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| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=27105086&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 27105086 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 1025028X 2DU0 jtl: Revista VacciMonitor (Vacunología y Temas Afines) issn: 1025028X maglogo: N pubinfo: dt: Oct2002 vid: 11 iid: 4 pid: 30675 pub: Vacunas Finlay S.A. artinfo: ui: 27105086 ppf: 1 ppct: 1 formats: fmt: @attributes: type: P size: 17KB tig: atl: PEROXIDASE-LABELED STICHOLYSINS METHOD FOR LOCALIZATION OF STICHOLYSINS IN THE SEA ANEMONE STICHODACTYLA HELIANTHUS. aug: au: Pico, M. C. Noa, Y. Pérez, V. Izquierdo, M. Guerra, O. Infante, J. F. Otero, A. affil: Centro de Estudio de Proteínas, Facultad de Biología, Universidad de La Habana. 25 No. 455 esq a J, Vedado, Ciudad Habana, Cuba Instituto Finlay. Ave 27 # 19807, Marianao, Ciudad Habana, Cuba Centro de Investigaciones de la Defensa Civil (CIDC). Carretera de Tapaste y 8 Vías, San José de las Lajas, Prov. Habana, Cuba su: Peroxidase Immunoenzyme technique Peptides Stichodactylidae Sea anemones Venom sug: subj: Peroxidase Immunoenzyme technique Peptides Stichodactylidae Sea anemones Venom ab: Sticholysins I and II are the major cytolysins present in the venom of the sea anemone Stichodactyla helianthus. These polypeptides have been isolated, purified and characterized (Lanio et al. 2001), and their immunogenic and antigenic properties studied (Pico et al. 2000). Anemones were collected in Miramar Coast, Ciudad Habana, fixed "in situ" in 4 % formaldehyde and 0.1 % glutaraldehyde, cut in small pieces and embedded in paraffin. Pieces were sliced to sections of 3 ?m, mounted on glass slides with polylysine, and then deparaffinized and rehydrated through xylene and a graded series of ethyl alcohol. To inhibit endogenous peroxidase sections were incubated for 10 min with 0.3 % hydrogen peroxide en methanol. Previously to immunohistochemistry stain samples were blocked 30 min with 1 % Triton X-100 in PBS and following incubated overnight with anti-sticholysins antibodies (0.25 ? g/mL) in PBS with 0.1 % Triton X-100 (PBS-TX). Afte r three 10 min rinses in PBS-TX the samples were incubated for 2 hours with peroxidase-labeled sticholysins diluted 1:500 in PBS-TX. Finally, the sections were washed 3 times and peroxidase activity was revealed by diaminobencidine. Control was performed replacing the anti-sticholysins by rabbit non-specific IgG. Using this method we can localize sticholysins in tentacles of anemone. The presence of the sticholysins in the tentacles suggests a role of these cytolysins in defense or prey capture processes of anemone. Lanio, M.E., Morera, V., Alvarez, C., Tejuca, M., Gómez, T., Pazos, F., Besada, V., Martínez, D., Huerta, V., Padrón, G. and Chávez, M.A. (2001) Purification and characterization of two hemolysins from Stichodactyla helianthus. Toxicon 39:187-194. Pico, M.C., del Monte, A., Calvo, L., Torres, Y., Felicó, E. and Otero, A.J. (2000) Inmunogenicidad y especificidad antigénica de las sticholisinas I y II de la anémona de mar Stichodactyla helianthus. Revista Biología 14(2):156-59. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista VacciMonitor (Vacunología y Temas Afines) is the property of Vacunas Finlay S.A. and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista VacciMonitor (Vacunología y Temas Afines) holder: Vacunas Finlay S.A. dt: @attributes: year: 2002 holdings: @attributes: islocal: N |
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