EXPERIMENTAL MURINE CRYPTOCOCCOSIS.

Cryptococcus neoformans is an encapsulated yeast-like organism which infects both immunologically competent and immunocompromised subjects . Cryptococcosis ranks in the top four infectious diseases causing death among patients with AIDS. In order to know the pathogenic potential of some cryptococcal...

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Publicado en:Revista VacciMonitor (Vacunología y Temas Afines) Vol. 11; no. 4; pp. 1 - 2
Autores principales: Milán, Odalis Asín, Machín, Gerardo Martínez, Ilnaitt, Maria Teresa, Valdez, Ileana
Formato: Artículo
Publicado: Vacunas Finlay S.A. Oct2002
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Acceso en línea:Ver este registro en EBSCOhost
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        atl: EXPERIMENTAL MURINE CRYPTOCOCCOSIS.
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        au:
          Milán, Odalis Asín
          Machín, Gerardo Martínez
          Ilnaitt, Maria Teresa
          Valdez, Ileana
        affil: Department of Bacteriology -- Mycology. Institute of Tropical Medicine "Pedro Kourí" Autopista Novia del Mediodia km 6. Habana City, Cuba. PO.Box 601, Marianao 13
      su:
        Cryptococcus neoformans
        AIDS patients
        Cryptococcales
        Strain theory (Chemistry)
        Animal disease models
      sug:
        subj:
          Cryptococcus neoformans
          AIDS patients
          Cryptococcales
          Strain theory (Chemistry)
          Animal disease models
      ab: Cryptococcus neoformans is an encapsulated yeast-like organism which infects both immunologically competent and immunocompromised subjects . Cryptococcosis ranks in the top four infectious diseases causing death among patients with AIDS. In order to know the pathogenic potential of some cryptococcal strains and to evaluate a murine mouse model of cryptococcosis, four groups of Balb/c mice (CENPALAB) with 20-24 g body weigh were anesthetized whit ketamina 150 mg/kg intraperitoneally and inoculated by intratracheal route with an inoculum of 10 cells of C. neoformans var. neoformans ( strains LMIPK 028, LMIPK 082 and LMIPK 089) in 50 µL of physiological saline solution (FSS) . These strains were isolated from patients with AIDS associated cryptococcosis. The control group was injected with 50 µL of FSS by the same route. The CFU assay in lungs, spleens and brain on days 9, 23, 35 y 42 after infection revealed a significant (p<0.05) difference between the animals infected with the strains LMIPK 082 when compared to animals infected with strains LMIPK 028 y LMIPK 089.. There was no discrepancy between latex test and CFU assay. The histopathology picture show a classical granulomatous lesion in lungs of animals infected with strains LMIPK 028 and LMIPK 089. The disease caused by the strains LMIPK 028 and LMIPK 089 in a mouse model of criptococcosis by intratracheal route is similar to that seen in immunocompromised patients. The mouse model of cryptococcosis is an efficient experimental model that produce disease with a similar spectrum of pathology to the disease in humans.
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