Immunomagnetic Diffractometry for Detection of Diagnostic Serum Markers.
We describe an integrated approach for detection of diagnostic markers using in situ assembled optical diffraction gratings in combination with immunomagnetic capture. Folate receptor (FR), a serum protein indicative of various cancers, was chosen as a model system to demonstrate the potential of th...
| Publicado en: | Journal of the American Chemical Society Vol. 129; no. 51; pp. 15824 - 15830 |
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| Autores principales: | , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
12/26/2007
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=28843167&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 28843167 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 12/26/2007 vid: 129 iid: 51 pid: 997 pub: American Chemical Society artinfo: ui: 28843167 10.1021/ja073094m ppf: 15824 ppct: 6 formats: tig: atl: Immunomagnetic Diffractometry for Detection of Diagnostic Serum Markers. aug: au: Acharya, Ghanashyam Chun-Li Chang Doorneweerd, Derek D. VIashi, Erina Henne, Walter A. Hartmann, Lynn C. Low, Philip S. Savran, Cagri A. affil: Birck Nanotechnology Center, Purdue University, West Lafayette, Indiana 47907 School of Electrical and Computer Engineering, Purdue University, West Lafayette, Indiana 47907 Department of Chemistry, Purdue University, West Lafayette, Indiana 47907 Department of Oncology, Mayo Clinic, Rochester, Minnesota 55905 School of Mechanical Engineering, Purdue University, West Lafayette, Indiana 47907 Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana 47907 su: Optical diffraction Vitamin B complex Blood proteins Cancer diagnosis Immunoglobulins Physical & theoretical chemistry sug: subj: Optical diffraction Vitamin B complex Blood proteins Cancer diagnosis Immunoglobulins Physical & theoretical chemistry ab: We describe an integrated approach for detection of diagnostic markers using in situ assembled optical diffraction gratings in combination with immunomagnetic capture. Folate receptor (FR), a serum protein indicative of various cancers, was chosen as a model system to demonstrate the potential of the method. Magnetic beads coupled to FR antibody were used to capture FR from serum. The FR-bound magnetic beads self-assembled onto microcontact-printed folate-coupled BSA (F-BSA) patterns to form diffraction gratings which served to detect FR by measuring the diffraction intensities caused by laser illumination. The FR-containing beads, upon binding to the F-BSA surface, served as intrinsic signal enhancement agents, circumventing the need for additional enzymatic signal amplification or fluorescent labeling steps. With this approach, a detection sensitivity of 700 fM (20 pg/mL) was achieved. The potential use of this approach in clinical diagnostics was demonstrated by measuring FR concentration in blood samples obtained from cancer patients. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2007 holdings: @attributes: islocal: N |
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