Subdiffraction Imaging through the Selective Donut-Mode Depletion of Thermally Stable Photoswitchable Fluorophores: Numerical Analysis and Application to the Fluorescent Protein Dronpa.
The fast and reversible on/off switching of the fluorescence emission of the GFP-like fluorescent protein Dronpa has attracted considerable interest for applications in subdiffraction imaging. In this paper we study the use of a donut-mode beam in combination with two more overlapping laser beams to...
| Publicado en: | Journal of the American Chemical Society Vol. 129; no. 51; pp. 16132 - 16142 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
12/26/2007
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=28843203&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 28843203 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 12/26/2007 vid: 129 iid: 51 pid: 997 pub: American Chemical Society artinfo: ui: 28843203 10.1021/ja076128z ppf: 16132 ppct: 10 formats: tig: atl: Subdiffraction Imaging through the Selective Donut-Mode Depletion of Thermally Stable Photoswitchable Fluorophores: Numerical Analysis and Application to the Fluorescent Protein Dronpa. aug: au: Dedecker, Peter Hotta, Jun-ichi FIors, Cristina Sliwa, Michel Uji-i, Hiroshi Roeffaers, Maarten B. J. Ando, Ryoko Mizuno, Hideaki Miyawaki, Atsushi Hofkens, Johan affil: Department of Chemistry and INPAC, Katholieke Universiteit Leuven, Celestijnenlaan 200 F, 3001 Heverlee, Belgium Brain Science Institute, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan su: Numerical analysis Green fluorescent protein Fluorescence Laser beams Optical resolution Optical diffraction sug: subj: Numerical analysis Green fluorescent protein Fluorescence Laser beams Optical resolution Optical diffraction ab: The fast and reversible on/off switching of the fluorescence emission of the GFP-like fluorescent protein Dronpa has attracted considerable interest for applications in subdiffraction imaging. In this paper we study the use of a donut-mode beam in combination with two more overlapping laser beams to increase the imaging resolution through selective switching to the nonfluorescent photoswitched state. We devise and run a series of numerical simulations to determine suitable photophysical parameters of prospective, thermally stable photoswitchable molecules, in terms of photoswitching quantum yields, fatigue resistance, and possible presence of transient nonfluorescent states. Many of our findings are applicable to other measurements that make use of donut beams, and these guidelines can be used in the synthesis and screening of novel photoswitchable compounds. We experimentally demonstrate the possibility of obtaining increased resolution by making use of the efficient and thermally stable Dronpa photoswitching, using equipment that is commonly available. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2007 holdings: @attributes: islocal: N |
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