Regeneration of the dog's axotomized sciatic nerve with pregnenolone-saturated chitosan prosthesis implanted through the tubulization technique.
Peripheral nerves can be injured by traumatisms or mechanical causes, and thermal, ischemic or tumoral damage, this could present inconveniences of the mobility, sensibility and loss of motor function at the denervated area. The surgical techniques applied to repair nerves have gone through several...
| Publicado en: | Veterinaria México Vol. 39; no. 1; pp. 55 - 65 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
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Facultad de Medicina Veterinaria y Zootecnia UNAM
ene-mar2008
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=31706530&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 31706530 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 03015092 7LD jtl: Veterinaria México issn: 03015092 maglogo: N pubinfo: dt: ene-mar2008 vid: 39 iid: 1 pid: 20869 pub: Facultad de Medicina Veterinaria y Zootecnia UNAM artinfo: ui: 31706530 ppf: 55 ppct: 10 formats: fmt: @attributes: type: P size: 4.3MB tig: atl: Regeneration of the dog's axotomized sciatic nerve with pregnenolone-saturated chitosan prosthesis implanted through the tubulization technique. aug: au: Rosales-Cortés, Manuel Peregrina-Sandoval, Jorge Hernández-Mercado, Jorge Nolasco-Rodríguez, Guillermo Chávez-Delgado, María Estela Gómez-Pinedo, Ulises Albarrán-Rodríguez, Esther affil: Laboratorio de Morfofisiología, Departamento de Medicina Veterinaria, División de Ciencias Veterinarias, Centro Universitario de Ciencias Biológicas Agropecuarias, Universidad de Guadalajara Km. 15.5, Carretera Guadalajara-Nogales, predio Las Agujas, Zapopan, Jalisco, México Laboratorio de Inmunologia, Departamento de Biologia Molecular, División de Ciencias Biológicas y Ambientales, Centro Universitario de Ciencias Biológicas Agropecuarias, Universidad de Guadalajara Km. 15.5, Carretera Guadalajara-Nogales, predio Las Agujas, Zapopan, Jalisco, México Departamento de Otorrinolaringología del Hospital General de Zona 89, Instituto Mexicano del Seguro Social, Guadalajara, Jalisco, México División de Patología y Biotecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del estado de Jalisco, A. C. y Laboratorio de Patología Clínica, OPD, Antiguo Hospital Civil de Guadalajara, Guadalajara, Jalisco, México su: Biomedical materials Chitosan Sciatic nerve Regeneration (Biology) Peripheral nervous system Pregnenolone sug: subj: Biomedical materials Chitosan Sciatic nerve Regeneration (Biology) Peripheral nervous system Pregnenolone keyword: AXOTOMIZED SCIATIC NERVE BIOMATERIAL CHITOSAN PERIPHERAL NERVES AND PREGNENOLONE REGENERATION ab: Peripheral nerves can be injured by traumatisms or mechanical causes, and thermal, ischemic or tumoral damage, this could present inconveniences of the mobility, sensibility and loss of motor function at the denervated area. The surgical techniques applied to repair nerves have gone through several phases of evolution such as the tubulization technique which consists of a prosthesis with a pipe shape at the segment injured. In this work the biomaterial utilized to manufacture the prosthesis was chitosan, since this compound allows to incorporate to its matrix promoting nervous growing substances that are released in situ for a long time while being degraded by titular lysosomes of organic origin. It is known, by diverse studies, that several neurosteroids are involved during the regeneration process of peripheral nerves, which functions are already described, as pregnenolone. In order to determine if there was or not regeneration and the degree of maturity of this ,12 young French Poodle female dogs of 1 to 1.5 years old were used (four were used as intact control group, four were subjected to tubulization technique with chitosan, and four went into tubulization technique with chitosan and pregnenolone neurosteroid, which is a stem hormone), in order to correct 15 mm of axotomized segment. The regenerated nerves were evaluated by means of electronic microscopy of transmission and light, performing cross cuts of 60-70 nm and 1 µm in thickness for their histological analysis. The morphological findings showed a similar structure to that of an intact nerve, since the number of myelinated axons, not myelinated and the proportion "g" (which indicates the ripening degree of the axon), were similar, indicating that the regeneration of the axotomized nerves and tubulization was achieved, independently of the administered treatment. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Veterinaria México is the property of Facultad de Medicina Veterinaria y Zootecnia UNAM and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Veterinaria México holder: Facultad de Medicina Veterinaria y Zootecnia UNAM dt: @attributes: year: 2008 holdings: @attributes: islocal: N |
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