Identification of leptin-induced transcripts in the mouse hypothalamus.
Long-form leptin receptor (OB-R(L)) is a signal-transducing member of the cytokine receptor superfamily that is essential for mediating the effects of leptin on mammalian body weight homeostasis. At present, the range of transcriptional targets responsive to OB-R(L) activation, and consequently, the...
| Publicado en: | Diabetes Vol. 49; no. 9; pp. 1443 - 1451 |
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| Autores principales: | , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American Diabetes Association
Sep2000
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=3539647&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 3539647 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00121797 1G6 jtl: Diabetes issn: 00121797 maglogo: N pubinfo: dt: Sep2000 vid: 49 iid: 9 pid: 1367 pub: American Diabetes Association place: Arlington, Virginia artinfo: ui: 3539647 3539647 NLM10969827 3539647 10.2337/diabetes.49.9.1443 NLM10969827 3539647 ppf: 1443 ppct: 8 formats: tig: atl: Identification of leptin-induced transcripts in the mouse hypothalamus. aug: au: White, David W. Zhou, Jianghong Stricker-Krongrad, Alain Ge, Pei Morgenstern, Jay P. Dembski, Marlene Tartaglia, Louis A. White, D W Zhou, J Stricker-Krongrad, A Ge, P Morgenstern, J P Dembski, M Tartaglia, L A affil: Millennium Pharmaceuticals, Cambridge, Massachusetts 02139-2406, USA sug: subj: Brain Metabolism Hypothalamus Metabolism Carrier Proteins RNA Drug Effects Neurons Metabolism Receptors, Cell Surface Leptin Pharmacodynamics Recombinant Proteins Viruses Male Animal Studies Genes Drug Effects Genetic Techniques Immunity Mice In Vitro Studies Cell Line Genes Validation Studies Comparative Studies Evaluation Research Multicenter Studies Male ab: Long-form leptin receptor (OB-R(L)) is a signal-transducing member of the cytokine receptor superfamily that is essential for mediating the effects of leptin on mammalian body weight homeostasis. At present, the range of transcriptional targets responsive to OB-R(L) activation, and consequently, the likely mediators of leptin action, remain undefined. In this report, we have used cDNA subtractive hybridization to identify transcripts induced by leptin in immortalized hypothalamic neurons expressing OB-R(L). Differential expression of the identified transcripts in these cells was confirmed by both array technology and Northern blotting. In situ hybridization studies indicate that these transcripts are expressed in the mouse central nervous system, including nuclei of the hypothalamus that coexpress OB-R(L). Comparative in situ analysis of slices of hypothalami generated from control and leptin-injected ob/ob mice demonstrates that a subset of the identified transcripts is induced in vivo after leptin injection. The potential role of the proteins encoded by these transcripts in mediating the effects of leptin on body weight and energy homeostasis are discussed. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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