Frequency and characterization of vapD gene in Helicobacter pylori strains of different vacA and cag-PAI genotype.

Several studies using different molecular-typing techniques have demonstrated that the gene content and the chromosomal arrangement among Helicobacter pylori strains are highly variable. Furthermore, this variability has been observed in different genes, such as vacA, which has a mosaic structure, a...

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Publicado en:Revista Neurologia, Neurocirugia y Psiquiatria Vol. 41; no. 2; pp. 43 - 51
Autores principales: Morales-Espinosa, Rosario, González-Valencia, Gerardo, Delgado, Gabriela, Méndez, José Luis, Torres, Javier, Cravioto, Alejandro
Formato: Artículo
Publicado: Sociedad Mexicana de Neurologia y Psiquiatria A.C. abr-jun2008
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Acceso en línea:Ver este registro en EBSCOhost
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        atl: Frequency and characterization of vapD gene in Helicobacter pylori strains of different vacA and cag-PAI genotype.
      aug:
        au:
          Morales-Espinosa, Rosario
          González-Valencia, Gerardo
          Delgado, Gabriela
          Méndez, José Luis
          Torres, Javier
          Cravioto, Alejandro
        affil:
          Lab of Molecular Epidemiological and Bacterial Genomic. Department of Microbiology and Parasitology
          Unit of Medical Investigation in Infectious and Parasitological Diseases, Instituto Mexicano del Seguro Social, XXI. Mexico City, Mexico
          Department of Public Health. Faculty of Medicine, Universidad Nacional Autónoma de México. Mexico City
      su:
        Helicobacter pylori
        Infection
        Genes
        Abdominal pain
        Mexico
      sug:
        subj:
          Mexico
          Helicobacter pylori
          Infection
          Genes
          Abdominal pain
      keyword:
        cag-PAI
        multiple infections
        vacA
        vapD typing
        cag-PAI
        genotipos de vacA
        Helicobacter pylori
        infección múltiple
        vapD
      ab:
        Several studies using different molecular-typing techniques have demonstrated that the gene content and the chromosomal arrangement among Helicobacter pylori strains are highly variable. Furthermore, this variability has been observed in different genes, such as vacA, which has a mosaic structure, and in cag-PAI, which has a different chromosomal organization. Preliminary study suggests that vapD is a strain-variable gene and it is present in about 60% of H. pylori strains. vapD gene is closely related to the virulence-associated protein D gene of Dichelobacter nodosus. The aim of this study was to characterize and to determine the frequency of vapD gene, in 301 Helicobacter pylori strains with different vacA and cag-PAI genotypes isolated from 27 Mexican patients with different gastric pathologies. The results showed that 70% of the patients presented multiple infections with H. pylori strains of different, vacA, and cag-PAI genotypes. Fifty two percent of the patients were colonized with vapD-positive and -negative strains. Regarding the vapD gene characterization by individual strains, it presented a frequency of 38%. This is the first characterization report that shows the frequency of vapD gene in H. pylori strains from Mexican patients. With respect to vacA and cag-PAI, the s1-m1/cag-PAI positive genotypes were more frequent in strains isolated from patients with recurrent abdominal pain (RAP), gastric ulcer (GU), and duodenal ulcer (DU). Statistical analysis showed that the vapD gene did not have association with any specific family of vacA or cag-PAI genotypes and specific gastric pathology.
        Diversos estudios, realizados con diferentes técnicas de tipificación molecular han demostrado que el contenido genético y arreglo cromosómico entre cepas de Helicobacter pylori son altamente variables. Además, se ha observado esta variabilidad en diferentes genes, como vacA que presenta una estructura de mosaico y cag-PAI, que presenta una organización diferente en el cromosoma. Un estudio preliminar sugiere que el gen vapD, es un gen variable de cepa, presente en aproximadamente 60% de las cepas de H. pylori. El gen vapD está relacionado con el gen de la proteína D asociada a virulencia de Dichelobacter nodosus. El objetivo de este estudio fue caracterizar y determinar la frecuencia del gen vapD en 301 cepas de H. pylori con diferentes genotipos de vacA y cag-PAI, provenientes de 27 pacientes con diferentes patologías gástricas. Los resultados mostraron que el 70% de los pacientes presentan infección múltiple con cepas de H. pylori de diferentes genotipos de vacA y cag-PAI. El 52% de ellos estuvieron colonizados con cepas vapD positivas y vapD negativas. La caracterización de vapD en cepas individuales, mostró una frecuencia del 38%. Este es el primer estudio que muestra la frecuencia de vapD en cepas de H. pylori aisladas de pacientes mexicanos. Con respecto a los genes vacA y cag-PAI, el genotipo más frecuente entre las cepas fue s1-m1/cag-PAI+, aisladas de pacientes con dolor abdominal crónico, úlcera gástrica y úlcera duodenal. El gen vapD no tiene asociación significativa con un alelo específico de vacA o el genotipo de cag-PAI y la patología gástrica.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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