Molecular identification and susceptibility testing of Trichosporon isolates from a Brazilian hospital.

In this study the molecular identification and susceptibility profile of 21 clinical isolates, from a Brazilian hospital, belonging to six different species of Trichosporon were described. Trichosporon asahii was the predominant species and corresponded to 43% of isolates followed by Trichosporon fa...

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Publicado en:Revista Iberoamericana de Micologia Vol. 25; no. 4; pp. 221 - 226
Autores principales: Ribeiro, Mariceli Araujo, Alastruey-Izquierdo, Ana, Gomez-Lopez, Alicia, Rodriguez-Tudela, Juan Luis, Cuenca-Estrella, Manuel
Formato: Artículo
Publicado: Elsevier B.V. dic2008
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Acceso en línea:Ver este registro en EBSCOhost
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        atl: Molecular identification and susceptibility testing of Trichosporon isolates from a Brazilian hospital.
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          Ribeiro, Mariceli Araujo
          Alastruey-Izquierdo, Ana
          Gomez-Lopez, Alicia
          Rodriguez-Tudela, Juan Luis
          Cuenca-Estrella, Manuel
        affil:
          Nucleo de Doenças Infecciosas, Universidade Federal do Espirito Santo, Brazil
          Servicio de Micología, Centro Nacional de Microbiología, Istituto de Salud Carlos III, Majadahonda, Madrid, Spain
      su:
        Yeast
        Microbial sensitivity tests
        Amphotericin B
        Effect of antibiotics on microorganisms
        Drug resistance in microorganisms
      sug:
        subj:
          Yeast
          Microbial sensitivity tests
          Amphotericin B
          Effect of antibiotics on microorganisms
          Drug resistance in microorganisms
      keyword:
        Antifungal susceptibility
        Molecular identification
        Trichosporon asahii
        Trichosporon inkin
        Identificación molecular
        Sensibilidad a los antifúngicos
        Trichosporon asahii
        Trichosporon inkin
      ab:
        In this study the molecular identification and susceptibility profile of 21 clinical isolates, from a Brazilian hospital, belonging to six different species of Trichosporon were described. Trichosporon asahii was the predominant species and corresponded to 43% of isolates followed by Trichosporon faecale (24%) and Trichosporon inkin (14%). Eighty three percent of the strains isolated from deep sites were identified as T. asahii, while only 17% belong to a non-T, asahii species (Trichosporon inkin). In general, the MICs were high and independent of the species of Trichosporon as well as the clinical origin of strain. Amphotericin B, 5-fluorocytosine and fluconazole were the less active against Trichosporon spp. and high MIC values of voriconazole, posaconazole and ravuconazole were observed. Fifty-six percent (5/9) of T. asahii strains were isolated from deep sites, whereas 8% (1/12) of non-T, asahii strains were isolated from those sites. A total of 89% of T. asahii isolates exhibited resistance to amphotericin B in vitro.
        En este artículo se describe la identificación molecular y el perfil de sensibilidad de 21 cepas clínicas pertenecientes a seis especies diferentes del género Trichosporon. La especie predominante fue Trichosporon asahii, que supuso el 43% de los aislamientos, seguido de Trichosporon faecale (24%) y Trichosporon inkin (14%). El 83% de las cepas aisladas de lugares anatómicos profundos fueron identificadas como T. asahii, mientras que sólo un 17% pertenecian a especies no-T, asahii. En general, los valores de CMI fueron altos, independientemente de la especie de Trichosporon y el origen clínico de la cepa. Los antifúngicos menos activos frente a Trichosporon fueron anfotericina B, fluorocitosina y fluconazol, aunque también se observaron valores altos de CMI a voriconazol, posaconazol y ravuconazol. El cincuenta y seis por ciento (5/9) de las cepas de T. asahii fueron aisladas de muestras profundas, mientas que el 8% (1/12) de las especies no-T, asahii fueron aisladas de dichos lugares. El 89% de los aislamientos de T. asahii mostraron resistencia in vitro a la anfotericina B.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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