Impaired oxidation of plasma-derived fatty acids in type 2 diabetic subjects during moderate-intensity exercise.

The present study was intended to investigate the different components of fatty acid utilization during a 60-min period of moderate-intensity cycling exercise (50% of VO2max) in eight male type 2 diabetic subjects (aged 52.6 +/- 3.1 years, body fat 35.8 +/- 1.3%) and eight male obese control subject...

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Publicado en:Diabetes Vol. 49; no. 12; pp. 2102 - 2108
Autores principales: Blaak, Ellen E., Blaak, E E, van Aggel-Leijssen, D P, Wagenmakers, A J, Saris, W H, van Baak, M A
Formato: research Journal Article
Publicado: American Diabetes Association Dec2000
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Dec2000
      vid: 49
      iid: 12
      pid: 1367
      pub: American Diabetes Association
      place: Arlington, Virginia
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        10.2337/diabetes.49.12.2102
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        atl: Impaired oxidation of plasma-derived fatty acids in type 2 diabetic subjects during moderate-intensity exercise.
      aug:
        au:
          Blaak, Ellen E.
          Blaak, E E
          van Aggel-Leijssen, D P
          Wagenmakers, A J
          Saris, W H
          van Baak, M A
        affil: Department of Human Biology, Nutrition Research Centre, Maastricht University, The Netherlands
      sug:
        subj:
          Fatty Acids Metabolism
          Diabetes Mellitus, Type 2 Blood
          Exercise
          Oxygen Consumption
          Reference Values
          Insulin Blood
          Human
          Calorimetry
          Fatty Acids Blood
          Arteries
          Obesity Complications
          Male
          Diabetes Mellitus, Type 2 Complications
          Oxidation-Reduction
          Blood Glucose Analysis
          Middle Age
          Osmolar Concentration
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Exercise of Self-Care Agency Scale
          Middle Aged: 45-64 years
          Male
      ab: The present study was intended to investigate the different components of fatty acid utilization during a 60-min period of moderate-intensity cycling exercise (50% of VO2max) in eight male type 2 diabetic subjects (aged 52.6 +/- 3.1 years, body fat 35.8 +/- 1.3%) and eight male obese control subjects (aged 45.1 +/- 1.4 years, body fat 34.2 +/- 1.3%) matched for age, body composition, and maximal aerobic capacity. To quantitate the different components of fatty acid metabolism, an isotope infusion of [U-13C]-palmitate was used in combination with indirect calorimetry. In separate experiments, the 13C label recovery in expired air was determined during infusion of [1,2-13C]-acetate (acetate recovery factor). There were no differences in energy expenditure or carbohydrate and total fat oxidation between the groups. The rate of appearance (Ra) of free fatty acid (FFA) (P < 0.05) and the exercise-induced increase in Ra of FFA were significantly lower (P < 0.05) in type 2 diabetic subjects compared with control subjects (baseline vs. exercise [40-60 min]; type 2 diabetes 11.9 +/- 0.9 vs. 19.6 +/- 2.2 micromol x kg(-1) fat-free mass [FFM] x min(-1) and control 15.8 +/- 1.8 vs. 28.6 +/- 2.1 micromol x kg(-1) FFM x min(-1)). The oxidation of plasma-derived fatty acids was significantly lower in type 2 diabetic subjects during both conditions (P < 0.05, baseline vs. exercise [40-60 min]; type 2 diabetes 4.2 +/- 0.5 vs. 14.1 +/- 1.9 micromol x kg(-1) FFM x min(-1) and control 6.2 +/- 0.6 vs. 20.4 +/- 1.9 micromol x kg(-1) FFM x min(-1)), whereas the oxidation of triglyceride-derived fatty acids was higher (P < 0.05). It is hypothesized that these impairments in fatty acid utilization may play a role in the etiology of skeletal muscle and hepatic insulin resistance.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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