Evaluation of a new immunochromatographic test for Helicobacter pylori IgG antibodies in elderly symptomatic patients.
Aim: To determine the diagnostic value of a new serum and whole blood serological IgG antibody test, FlexPack HP, for the diagnosis of Helicobacter pylori in elderly symptomatic patients.Methods: 94 consecutive symptomatic patients who underwent upper endoscopy were studied (mean age, 62.6 years). O...
| Publicado en: | Journal of Gastroenterology Vol. 34; no. 1; pp. 7 - 11 |
|---|---|
| Autores principales: | , , , , , , , , , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Springer Nature
1999
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=4690108&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 4690108 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09441174 EJ7 jtl: Journal of Gastroenterology issn: 09441174 maglogo: N pubinfo: dt: 1999 vid: 34 iid: 1 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 4690108 4690108 NLM10204604 4690108 10.1007/s005350050209 NLM10204604 4690108 ppf: 7 ppct: 4 formats: tig: atl: Evaluation of a new immunochromatographic test for Helicobacter pylori IgG antibodies in elderly symptomatic patients. aug: au: Shirin, Haim Bruck, Rafael Kenet, Gabriel Krepel, Zipora Wardi, Yoram Reif, Simon Zaidel, Liliana Geva, Dikla Avni, Yona Halpern, Zamir Shirin, H Bruck, R Kenet, G Krepel, Z Wardi, J Wardi, Y Reif, S Zaidel, L Geva, D Avni, Y affil: Department of Gastroenterology, The E. Wolfson Medical Center, Holon, Israel sug: subj: Immunoglobulins Analysis Gastritis Diagnosis Helicobacter Pylori Immunology Duodenal Ulcer Diagnosis Antibodies, Bacterial Analysis Helicobacter Infections Diagnosis Biopsy Middle Age Human Gastritis Microbiology Chromatography Duodenal Ulcer Blood Male Endoscopy, Digestive System Predictive Value of Tests Female Pyloric Antrum Microbiology Pyloric Antrum Pathology Helicobacter Infections Blood Aged Reproducibility of Results Immunoassay Helicobacter Infections Microbiology Gastritis Blood Duodenal Ulcer Microbiology Validation Studies Comparative Studies Evaluation Research Multicenter Studies Middle Aged: 45-64 years Aged: 65+ years Male Female ab: Aim: To determine the diagnostic value of a new serum and whole blood serological IgG antibody test, FlexPack HP, for the diagnosis of Helicobacter pylori in elderly symptomatic patients.Methods: 94 consecutive symptomatic patients who underwent upper endoscopy were studied (mean age, 62.6 years). On endoscopy, the presence of H. pylori infection was examined by biopsies from gastric antrum and body for rapid urease test and histologic examination. Blood was drawn prior to endoscopy and both blood and serum were immediately analyzed for human IgG antibodies to H. pylori by a new commercially available qualitative immunochromatographic method, FlexPack HP. This test incorporates high-molecular weight cell-associated proteins (HM-CAP), which are highly specific for H. pylori IgG antibodies.Results: Overall agreement for FlexPack HP whole blood vs FlexPack HP serum was 100%, and agreement with biopsy results was 71%. The gold standard (detection of H. pylori by histology or urease test) identified H. pylori in 61 patients (65%). Complete agreement was observed between the gold standard test and the serology kit in 72% (68/94) of sera (51 positive and 17 negative). Disagreement was found in sera of 26 patients; 16 sera were negative by the gold standard and positive by FlexPack HP and 10 patients were found negative by serology. The sensitivity of FlexPack HP was 84% and the specificity 52% when compared with the gold standard.Conclusions: FlexPack HP serum and whole blood test is a simple and reliable method for the detection of H. pylori antibodies, with 100% agreement between the serum and blood results. In the elderly symptomatic patients the sensitivity of FlexPack HP was similar to that of other serologic tests, but the specificity was relatively low, limiting its use in this population. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|