Multiplexed Imaging of Therapeutic Cells with Multispectrally Encoded Magnetofluorescent Nanocomposite Emulsions.
Here, we describe the fabrication of multispectrally encoded nanoprobes, perfluorocarbon (PFC)/quantum dots (QDs) nanocomposite emulsions, which could provide both multispectral MR and multicolor optical imaging modalities. Our strategy exploited the combination of the multispectral MR properties of...
| Publicado en: | Journal of the American Chemical Society Vol. 131; no. 47; pp. 17145 - 17155 |
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| Autores principales: | , , , , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
12/2/2009
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=46974682&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 46974682 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 12/2/2009 vid: 131 iid: 47 pid: 997 pub: American Chemical Society artinfo: ui: 46974682 10.1021/ja904472z ppf: 17145 ppct: 10 formats: tig: atl: Multiplexed Imaging of Therapeutic Cells with Multispectrally Encoded Magnetofluorescent Nanocomposite Emulsions. aug: au: Yong Taik Lim Young-Woock Noh Jee-Hyun Cho Jung Hyun Han Bang Sil Choi Jina Kwon Kwan Soo Hong Gokarna, Anisha Yong-Hoon Cho Bong Hyun Chung affil: BioNanorechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea MRI Team, Korea Basic Science Institute, Ochang, Chungbuk, South Korea Department of Physics, Korea Advanced Institute of Science and Technology, South Korea su: Imaging systems Spectrum analysis Perfluorocarbons Quantum dots Emulsions Mixtures sug: subj: Imaging systems Spectrum analysis Perfluorocarbons Quantum dots Emulsions Mixtures ab: Here, we describe the fabrication of multispectrally encoded nanoprobes, perfluorocarbon (PFC)/quantum dots (QDs) nanocomposite emulsions, which could provide both multispectral MR and multicolor optical imaging modalities. Our strategy exploited the combination of the multispectral MR properties of four different PFC materials and the multicolor emission properties of three different colored CdSe/ZnS QDs. The PFC/QDs nanocomposite emulsions were fabricated by exchanging hydrophobic ligands coated onto CdSe/ZnS QDs using 1H,1H,2H,2H-perfluorooctanethiol, which renders the QDs to be dispersible in the PFC liquids. To provide biocompatibility, the PFC liquids containing QDs were emulsified into aqueous solutions with the aid of phospholipids. The distinct 19F-based MR images of PFC/QDs nanocomposite emulsions were obtained by selective excitation of the nanocomposite emulsions with magnetic resonance frequency of each PFC, while a specific fluorescence image of them could be selected using appropriate optical filters. The uptake of PFC/QDs nanocomposite emulsions was high in phagocytic cells such as macrophages (90.55%) and dendritic cells (85.34%), while it was low in nonphagocytic I cells (33%). We have also shown that the nanocomposite emulsions were successfully applied to differentially visualize immunotherapeutic cells (macrophages, dendritic cells, and T cells) in vivo. The PFC/QDs nanocomposite emulsions are expected to be a promising multimodality nanoprobe for the multiplexed detection and imaging of therapeutic cells both in vitro and in vivo. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2009 holdings: @attributes: islocal: N |
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