Analysis of the parameters of the ejaculate in the laboratory Wistar rat: technical description.
The number of spermatozoa, weight and size of the seminal plug has been quantified in the ejaculate of the laboratory rat and sperm viability, sperm mobility and sperm concentration in samples obtained from the epididymis. The reference values for the rat ejaculate have not been determined maybe due...
| Publicado en: | Veterinaria México Vol. 40; no. 4; pp. 405 - 416 |
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| Autores principales: | , , , , |
| Formato: | Artículo |
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Facultad de Medicina Veterinaria y Zootecnia UNAM
oct-dic2009
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=47840449&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 47840449 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 03015092 7LD jtl: Veterinaria México issn: 03015092 maglogo: N pubinfo: dt: oct-dic2009 vid: 40 iid: 4 pid: 20869 pub: Facultad de Medicina Veterinaria y Zootecnia UNAM artinfo: ui: 47840449 ppf: 405 ppct: 11 formats: fmt: @attributes: type: P size: 863KB tig: atl: Analysis of the parameters of the ejaculate in the laboratory Wistar rat: technical description. aug: au: Lucio, Rosa Angélica Tlachi, José Luis López, Aurora Adriana Zempoalteca, René Velázquez-Moctezuma, Javier affil: Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Carretera Tlaxcala-Puebla, km 1.5 s/n, Tlaxcala, 90062, Tlaxcala, México, Apartado Postal 264 Maestría en Ciencias Biológicas, Universidad Autónoma de Tlaxcala, Carretera Tlaxcala-Puebla, km 1.5 s/n, Tlaxcala, 90062, Tlaxcala, México Área de Neurociencias, Universidad Autónoma Metropolitana-Iztapalapa, Avenida Michoacán y La Purísima, Iztapalapa, 38340, México, D. F. su: Male ejaculation Ejaculation Semen Sperm motility Rat reproduction Epididymis sug: subj: Male ejaculation Ejaculation Semen Sperm motility Rat reproduction Epididymis keyword: NUMBER OF SPERMATOZOA SEMEN SEMINAL PLUG SPERM MOBILITY SPERM MORPHOLOGY SPERM VIABILITY ab: The number of spermatozoa, weight and size of the seminal plug has been quantified in the ejaculate of the laboratory rat and sperm viability, sperm mobility and sperm concentration in samples obtained from the epididymis. The reference values for the rat ejaculate have not been determined maybe due to the difficulty to obtain it directly from the male. Nevertheless, an approach can be obtained analyzing the ejaculate collected from the inseminated female. The objective of this study was to evaluate and propose the values of the macroscospic and microscopic parameters of the semen and seminal plug obtained from the female. The seminal analysis of the Wistar rats was performed adapting the methods used for other species. After one ejaculatory series, semen contained in the uterine horns was obtained, as well as the seminal plug from the vagina. In more than one hundred ejaculates, 94.54% were off-white with 2.35 ± 0.06 mm of viscosity and 8.13 ± 0.02 of pH. The sperm concentration was 16.3 ± 0.59 millions of spermatozoa per mL, 0.72 ± 0.01 mobility index, 64.4 ± 0.7% viability and 99.11 ± 0.20% normal morphology. From 99% of seminal plugs, 92.7% were hardened, they weighed 115.63 ± 1.54 mg, measured 12.41 ± 0.13 and 5.31 ± 0.05 mm of length and width respectively, and volume 87.70 ± 1.74 mm3. In conclusion, the used method to obtain and to evaluate the parameters of the ejaculate is reliable, for that reason, it is suggested that the results obtained could be considered as indicative values for semen and seminal plug for the Wistar rat. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Veterinaria México is the property of Facultad de Medicina Veterinaria y Zootecnia UNAM and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Veterinaria México holder: Facultad de Medicina Veterinaria y Zootecnia UNAM dt: @attributes: year: 2009 holdings: @attributes: islocal: N |
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