Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation.
We described a hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species. A nanopipette/nanoring el...
| Publicado en: | Journal of the American Chemical Society Vol. 132; no. 29; pp. 10118 - 10127 |
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| Autores principales: | , , , , , , |
| Formato: | Artículo |
| Publicado: |
American Chemical Society
7/28/2010
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| Materias: | |
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=hlh&AN=52892860&site=ehost-live header: @attributes: shortDbName: hlh uiTerm: 52892860 longDbName: Humanities International Complete uiTag: AN controlInfo: bkinfo: jinfo: jid: 00027863 ACS jtl: Journal of the American Chemical Society issn: 00027863 maglogo: N pubinfo: dt: 7/28/2010 vid: 132 iid: 29 pid: 997 pub: American Chemical Society artinfo: ui: 52892860 10.1021/ja1029478 ppf: 10118 ppct: 9 formats: tig: atl: Simultaneous Noncontact Topography and Electrochemical Imaging by SECM/SICM Featuring Ion Current Feedback Regulation. aug: au: Takahashi, Yasufumi Shevchuk, Andrew I. Novak, Pavel Murakami, Yumi Shiku, Hitoshi Korchev, Yuri E. Matsue, Tomokazu affil: Graduate School of Environmental Studies, Tohoku University, Aramaki Aoba 6-6-11-605, Sendai 980-8579, Japan Division of Medicine, Imperial College London, Hammersmith Hospital Campus, London W12 0NN, United Kingdom su: Imaging systems Electrochemical analysis Scanning electrochemical microscopy Electrodes Enzymes Oxidases Muscle cells High resolution spectroscopy sug: subj: Imaging systems Electrochemical analysis Scanning electrochemical microscopy Electrodes Enzymes Oxidases Muscle cells High resolution spectroscopy ab: We described a hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species. A nanopipette/nanoring electrode probe provided submicrometer resolution of the electrochemical measurement on surfaces with complex topology. The SECM/SICM probe had an aperture radius of 220 nm. The inner and outer radii of the SECM Au nanoring electrode were 330 and 550 nm, respectively. Characterization of the probe was performed with scanning electron microscopy (SEM), cyclic voltammetry (CV), and approach curve measurements. SECM/SICM was applied to simultaneous imaging of topography and electrochemical responses of enzymes (horse radish peroxidase (HRP) and glucose oxidase (GOD)) and single live cells (A6 cells, superior cervical ganglion (SCG) cells, and cardiac myocytes). The measurements revealed the distribution of activity of the enzyme spots on uneven surfaces with submicrometer resolution. SECM/SICM acquired high resolution topographic images of cells together with the map of electrochemical signals. This combined technique was also applied to the evaluation of the permeation property of electroactive species through cellular membranes. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y dt: @attributes: year: 2010 holdings: @attributes: islocal: N |
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