Helicobacter pylori resistance to metronidazole, clarithromycin and amoxicillin in Colombian patients.
Helicobacter pylori (H. pylori), is a universal pathogen that infects more than half the world population. In the last two decades, the recommended treatment for its eradication, as first-line scheme is the standard triple therapy, consisting of an inhibitor of the proton pump, clarithromycin and am...
| Publicado en: | Revista Colombiana de Gastroenterología Vol. 25; no. 1; pp. 29 - 37 |
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| Autores principales: | , , |
| Formato: | Artículo |
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Asociacion Colombiana de Gastroenterologia
Mar2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=54024204&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 54024204 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 01209957 78D5 jtl: Revista Colombiana de Gastroenterología issn: 01209957 maglogo: N pubinfo: dt: Mar2010 vid: 25 iid: 1 pid: 47611 pub: Asociacion Colombiana de Gastroenterologia artinfo: ui: 54024204 ppf: 29 ppct: 8 formats: fmt: @attributes: type: P size: 148KB tig: atl: Helicobacter pylori resistance to metronidazole, clarithromycin and amoxicillin in Colombian patients. aug: au: Trespalacios, Alba Alicia Regino, William Otero Reyes, Marcela Mercado affil: Associate Professor and Medical Microbiology Coordinator, Department of Microbiology, Faculty of Sciences, Pontificia Universidad Javeriana. Bogotá, Colombia Associate Professor of Medicine, Gastroenterology Unit Coordinator, Universidad Nacional de Colombia. Gastroenterologist, Clinica Fundadores. Bogotá, Colombia Assistant Professor, Department of Microbiology, Faculty of Sciences, Pontificia Universidad Javeriana. Bogotá, Colombia su: Helicobacter pylori Drug resistance in microorganisms Metronidazole Amoxicillin Medical care Therapeutics Colombia sug: subj: Colombia Helicobacter pylori Drug resistance in microorganisms Metronidazole Amoxicillin Medical care Therapeutics keyword: amoxicillin clarithromycin genotypes metronidazole ab: Helicobacter pylori (H. pylori), is a universal pathogen that infects more than half the world population. In the last two decades, the recommended treatment for its eradication, as first-line scheme is the standard triple therapy, consisting of an inhibitor of the proton pump, clarithromycin and amoxicillin or metronidazole. In recent years the effectiveness of this therapy has declined, especially due to the resistance of bacteria to metronidazole and clarithromycin. Objectives: In this study, we evaluated the prevalence of primary resistance of Colombian H. pylori isolates to metronidazole, clarithromycin, amoxicillin. In addition, the vacA and cagA genotypes of strains isolated were determined and associated to correlate the virulence markers and antibiotic resistance. Methods: Minimum inhibitory concentration (MIC) for metronidazole, clarithromycin and amoxicillin were determined by E-test method. Genomic DNA was extracted, and allelic variants of vacA and cagA were identified by the polymerase chain reaction (PCR). Results: Resistance to metronidazole was 81.01 % (IC95% 70.3%-88.6%), to amoxicillin 3,8% (IC 95% 0-8,6%), and to clarithromycin 17.72% (IC95% 10.37-28.29). No significant correlation between pathogenicity and resistance or susceptibility was detected when MIC values for each antibiotic were compared with different vacA and cagA genotypes. Conclusion: We find a high rate of resistance to three principal antibiotics used in the majority of the successful schemes of eradication of the infection, which implies the need to investigate with priority new schemes of treatment for the eradication of the infection in Colombia. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Revista Colombiana de Gastroenterología is the property of Asociacion Colombiana de Gastroenterologia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Revista Colombiana de Gastroenterología holder: Asociacion Colombiana de Gastroenterologia dt: @attributes: year: 2010 holdings: @attributes: islocal: N |
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