Molecular Characterization of Putative Chordoma Cell Lines.
Immortal tumor cell lines are an important model system for cancer research, however,misidentification and cross-contamination of cell lines are a common problem. Seven chordoma cell lines are reported in the literature, but none has been characterized in detail. We analyzed gene expression patterns...
| Publicado en: | Sarcoma pp. 1 - 15 |
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| Autores principales: | , , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Wiley-Blackwell
2010
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=57209804&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 57209804 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1357714X 57R jtl: Sarcoma issn: 1357714X maglogo: Y pubinfo: dt: 2010 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 57209804 104979798 10.1155/2010/630129 57209804 ppf: 1 ppct: 14 formats: fmt: @attributes: type: P tig: atl: Molecular Characterization of Putative Chordoma Cell Lines. aug: au: Brüderlein, Silke Sommer, Joshua B. Meltzer, Paul S. Sufeng Li Osada, Takuya Ng, David Möller, Peter Alcorta, David A. Kelley, Michael J. affil: Institute of Pathology, Ulm University, D-89081 Ulm, Germany. sug: subj: Neoplasms, Germ Cell and Embryonal Pathology Gene Expression Neoplasms, Germ Cell and Embryonal Familial and Genetic Blotting, Western Human Aged Female Middle Age Male Descriptive Statistics Flow Cytometry Fluorescent Antibody Technique Genotype Funding Source Aged: 65+ years Middle Aged: 45-64 years Female Male ab: Immortal tumor cell lines are an important model system for cancer research, however,misidentification and cross-contamination of cell lines are a common problem. Seven chordoma cell lines are reported in the literature, but none has been characterized in detail. We analyzed gene expression patterns and genomic copy number variations in five putative chordoma cell lines (U-CH1, CCL3, CCL4, GB60, and CM319). We also created a new chordoma cell line, U-CH2, and provided genotypes for cell lines for identity confirmation. Our analyses revealed that CCL3, CCL4, and GB60 are not chordoma cell lines, and that CM319 is a cancer cell line possibly derived from chordoma, but lacking expression of key chordoma biomarkers. U-CH1 and U-CH2 both have gene expression profiles, copy number aberrations, and morphology consistent with chordoma tumors. These cell lines also harbor genetic changes, such as loss of p16, MTAP, or PTEN, that make them potentially useful models for studying mechanisms of chordoma pathogenesis and for evaluating targeted therapies. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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