Free radicals and antioxidants at a glance using EPR spectroscopy...electron paramagnetic resonance
The delicate balance between the advantageous and detrimental effects of free radicals is one of the important aspects of human (patho)physiology. The controlled production of reactive oxygen and nitrogen species has an essential role in the regulation of various signaling switches. On the other han...
| Published in: | Critical Reviews in Clinical Laboratory Sciences Vol. 48; no. 3; pp. 114 - 143 |
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| Format: | pictorial review tables/charts tracings Journal Article |
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Taylor & Francis Ltd
May/Jun2011
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=65076655&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 65076655 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10408363 1AV jtl: Critical Reviews in Clinical Laboratory Sciences issn: 10408363 maglogo: Y pubinfo: dt: May/Jun2011 vid: 48 iid: 3 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 65076655 65076655 65076655 10.3109/10408363.2011.591772 65076655 ppf: 114 ppct: 29 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Free radicals and antioxidants at a glance using EPR spectroscopy...electron paramagnetic resonance aug: au: Spasojević, Ivan affil: Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia sug: subj: Free Radicals Analysis Antioxidants Oxidative Stress Nitric Oxide Metabolism Oxidation-Reduction Physiology Free Radicals Adverse Effects Free Radicals Physiology Reactive Oxygen Species Reactive Nitrogen Species Superoxide Dismutase Dosimetry Chemistry, Analytical Methods ab: The delicate balance between the advantageous and detrimental effects of free radicals is one of the important aspects of human (patho)physiology. The controlled production of reactive oxygen and nitrogen species has an essential role in the regulation of various signaling switches. On the other hand, imbalanced generation of radicals is highly correlated with the pathogenesis of many diseases which require the application of selected antioxidants to regain the homeostasis. In the era of growing interest for redox processes, electron paramagnetic resonance (EPR) spectroscopy is arguably the best-suited technique for such research due to its ability to provide a unique insight into the world of free radicals and antioxidants. Herein, I present the principles of EPR spectroscopy and the applications of this method in assessing: (i) the oxidative status of biological systems, using endogenous long-lived free radicals (ascorbyl radical (Asc•), tocopheroxyl radical (TO•), melanin) as markers; (ii) the production of short-lived radicals (hydroxyl radical (OH•), superoxide radical anion (O2•−), sulfur- and carbon-centered radicals), which are implicated in both, oxidative stress and redox signaling; (iii) the metabolism of nitric oxide (NO•); (iv) the antioxidative properties of various drugs, compounds, and natural products; (v) other redox-relevant parameter. Besides giving a comprehensive survey of up-to-date literature, I also provide illustrative examples in sufficient detail to provide a means to exploit the potential of EPR in biochemical/physiological/medical research. The emphasis is on the features and characteristics (both positive and negative) relevant for EPR application in clinical sciences. My aim is to encourage fellow colleagues interested in free radicals and antioxidants to expand their base knowledge or methods used in their laboratories with data acquired by EPR or some of the EPR techniques outlined in this review, in order to boost up the exciting area of redox science. pubtype: Academic Journal doctype: pictorial review tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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