Time-Resolved Events on the Reaction Pathway of Transcript Initiation by a Single-Subunit RNA Polymerase: Raman Crystallographic Evidence.

The nucleotidyl transfer reaction leading to formation of the first phosphodiester bond has been followed in real time by Raman microscopy, as it proceeds in single crystals of the N4 phage virion RNA polymerase (RNAP). The reaction is initiated by soaking nucleoside triphosphate (NTP) substrates an...

Descripción completa

Detalles Bibliográficos

Publicado en:	Journal of the American Chemical Society Vol. 133; no. 32; pp. 12544 - 12556
Autores principales:	Yuanyuan Chen, Basu, Ritwika, Gleghorn, Michael L., Murakami, Katsuhiko S., Carey, Paul R.
Formato:	Artículo
Publicado:	American Chemical Society 8/17/2011
Materias:	RNA polymerases Raman effect Nucleosides Phosphodiesters DNA Enzymes
Acceso en línea:	Ver este registro en EBSCOhost