Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo.

Objective: The objective of this study was to assess the potential of 3 spectroscopic techniques (intrinsic fluorescence, diffuse reflectance, and light scattering) individually and in combination (trimodal spectroscopy) for the detection of cervical squamous intraepithelial lesions.Study Design: Th...

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Published in:American Journal of Obstetrics & Gynecology Vol. 186; no. 3; pp. 374 - 383
Main Authors: Georgakoudi, Irene, Sheets, Ellen E., Müller, Markus G., Backman, Vadim, Crum, Christopher P., Badizadegan, Kamran, Dasari, Ramachandra R., Feld, Michael S., Müller, Markus G
Format: research Journal Article
Published: Elsevier B.V. Mar2002
Online Access:View this record in EBSCOhost
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      dt: Mar2002
      vid: 186
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      pub: Elsevier B.V.
      place: New York, New York
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        10.1067/mob.2002.121075
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        atl: Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo.
      aug:
        au:
          Georgakoudi, Irene
          Sheets, Ellen E.
          Müller, Markus G.
          Backman, Vadim
          Crum, Christopher P.
          Badizadegan, Kamran
          Dasari, Ramachandra R.
          Feld, Michael S.
          Müller, Markus G
      sug:
        subj:
          Cervix Neoplasms Diagnosis
          Spectrum Analysis Methods
          Precancerous Conditions Diagnosis
          Human
          Scattering, Radiation
          Light
          Diagnosis, Differential
          Female
          Validation Studies
          Comparative Studies
          Evaluation Research
          Multicenter Studies
          Female
      ab: Objective: The objective of this study was to assess the potential of 3 spectroscopic techniques (intrinsic fluorescence, diffuse reflectance, and light scattering) individually and in combination (trimodal spectroscopy) for the detection of cervical squamous intraepithelial lesions.Study Design: The study was conducted with 44 patients who underwent colposcopy for the evaluation of an abnormal Papanicolaou smear. Fluorescence and reflectance spectra were collected from colposcopically normal and abnormal sites and analyzed to extract quantitative information about tissue biochemistry and morphologic condition. This information was compared with histopathologic classification, and diagnostic algorithms were developed and validated with the use of logistic regression and cross-validation.Results: Diagnostically significant differences exist in the composition of fluorescing biochemicals, the scattering properties, and the epithelial cell nuclear morphology of cervical squamous intraepithelial lesions and non-squamous intraepithelial lesions. Trimodal spectroscopy is a superior tool for the detection of cervical squamous intraepithelial lesions than any 1 of the techniques alone.Conclusion: Trimodal spectroscopy has the potential to improve the in vivo detection of precancerous cervical changes.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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