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Aberrant CpG Island Hypermethylation in Pediatric Gastric Mucosa in Association With Helicobacter pylori Infection.

Context.-Helicobacter pylori infection is primarily acquired during childhood and persists throughout life in the absence of eradication with antibiotics. Helicobacter pylori infection induces methylation in the promoter CpG island loci in gastric epithelial cells. Thus, aberrant CpG island hypermet...

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Published in:Archives of Pathology & Laboratory Medicine Vol. 135; no. 6; pp. 759 - 766
Main Authors: Shin, So-Hyun, Park, Seog-Yun, Ko, Jae-Sung, Kim, Nayoung, Kang, Gyeong Hoon
Format: research tables/charts Journal Article
Published: College of American Pathologists Jun2011
Online Access:View this record in EBSCOhost
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      dt: Jun2011
      vid: 135
      iid: 6
      pid: 2550
      pub: College of American Pathologists
      place: Northfield, Illinois
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        66931745
        66931745
        104637600
        10.5858/2010-0140-oa.1
        66931745
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      tig:
        atl: Aberrant CpG Island Hypermethylation in Pediatric Gastric Mucosa in Association With Helicobacter pylori Infection.
      aug:
        au:
          Shin, So-Hyun
          Park, Seog-Yun
          Ko, Jae-Sung
          Kim, Nayoung
          Kang, Gyeong Hoon
        affil: Laboratory of Epigenetics, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
      sug:
        subj:
          Gastric Mucosa Pathology
          Helicobacter Infections Familial and Genetic
          Helicobacter Infections Pathology
          Methylation
          Helicobacter Infections Metabolism
          Human
          Comparative Studies
          Child, Preschool
          Child
          Adolescence
          Adult
          Middle Age
          Female
          Male
          Glycoproteins
          Chemical Phenomena
          Neutrophils Pathology
          Sequence Analysis
          Oligonucleotide Array Sequence Analysis
          Data Analysis Software
          Analysis of Variance
          Mann-Whitney U Test
          T-Tests
          P-Value
          Chi Square Test
          Funding Source
          Child, Preschool: 2-5 years
          Child: 6-12 years
          Adolescent: 13-18 years
          Adult: 19-44 years
          Middle Aged: 45-64 years
          Female
          Male
      ab: Context.-Helicobacter pylori infection is primarily acquired during childhood and persists throughout life in the absence of eradication with antibiotics. Helicobacter pylori infection induces methylation in the promoter CpG island loci in gastric epithelial cells. Thus, aberrant CpG island hypermethylation in gastric epithelial cells likely occurs early in life, although there are no existing data supporting this notion. Objectives.-To identify whether aberrant CpG island hypermethylation occurs in pediatric stomach mucosa in association with H pylori infection and to compare methylation profiles of samples from pediatric and adult stomach tissues. Design.-We analyzed pediatric (n = 47) and adult (n = 38) gastric mucosa samples for their methylation status in 12 promoter CpG island loci using the MethyLight assay and compared the number of methylated genes and the methylation levels in individual genes between H pylori-positive and H pylori-negative sample results and between pediatric and adult samples. Results.-The average number of methylated genes was significantly higher in H pylori-infected pediatric samples than in H pylori-negative pediatric samples (3.4 versus 0.3, P < .001) and in H pylori-infected adult samples than in H pylori-negative adult samples (7.6 versus 0.9, P < .001). Seven genes showed significantly higher methylation levels in H pylori-infected pediatric samples than in H pylori-negative pediatric samples (all values were P < .05). Conclusions.-These results indicate that CpG island hypermethylation occurs in pediatric gastric mucosa in association with H pylori infection and that the genes affected by H pylori-associated hypermethylation were similar in pediatric and adult samples.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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