First assessment of needle-based confocal laser endomicroscopy during EUS-FNA procedures of the pancreas (with videos)

Background: Challenges in EUS-guided FNA (EUS-FNA) include sampling error, nondiagnostic cytology, and limited on-site cytological evaluation. A prototype needle-based confocal laser endomicroscopy (nCLE) probe is a submillimeter probe that provides real-time imaging at the microscopic level through...

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Publicado en:Gastrointestinal Endoscopy Vol. 74; no. 5; pp. 1049 - 1061
Autores principales: Konda, Vani J.A., Aslanian, Harry R., Wallace, Michael B., Siddiqui, Uzma D., Hart, John, Waxman, Irving
Formato: research Journal Article
Publicado: Elsevier B.V. Nov2011
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Nov2011
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      pub: Elsevier B.V.
      place: New York, New York
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        10.1016/j.gie.2011.07.018
        67013978
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        atl: First assessment of needle-based confocal laser endomicroscopy during EUS-FNA procedures of the pancreas (with videos)
      aug:
        au:
          Konda, Vani J.A.
          Aslanian, Harry R.
          Wallace, Michael B.
          Siddiqui, Uzma D.
          Hart, John
          Waxman, Irving
        affil: Center for Endoscopic Research and Therapeutics, Section of Gastroenterology, Department of Medicine, University of Chicago Medical Center, Chicago, Illinois, USA
      sug:
        subj:
          Endosonography Methods
          Biopsy, Needle Methods
          Pancreatic Diseases Diagnosis
          Human
          Videorecording
          Multicenter Studies
          Academic Medical Centers
          Treatment Outcomes
      ab: Background: Challenges in EUS-guided FNA (EUS-FNA) include sampling error, nondiagnostic cytology, and limited on-site cytological evaluation. A prototype needle-based confocal laser endomicroscopy (nCLE) probe is a submillimeter probe that provides real-time imaging at the microscopic level through the FNA needle. Objective: To evaluate the feasibility of nCLE during EUS-FNA of pancreatic lesions. Design: Feasibility study. Setting: Multicenter, tertiary care. Patients: Eighteen patients presenting for EUS-FNA. Interventions: Patients were injected with 2.5 mL of 10% fluorescein. The lesion was interrogated with the nCLE probe positioned at the tip of a 19-gauge FNA needle. Main Outcome Measurements: Device integrity, technical ease, safety, and image acquisition. Results: Cases included 16 cysts and 2 masses. There were no device malfunctions. Technical challenges were encountered in 6 of 18 attempts to image and reflected challenges with a postloading technique, the longer ferule tip, and a transduodenal approach. Technical feasibility to perform imaging with nCLE during a pancreatic EUS-FNA procedure was achieved in 17 of 18 cases. Ten cases had good to very good image quality. Two serious adverse events occurred; both were pancreatitis requiring hospitalization. Limitations: Limited sample size, small number of patients with confirmed pathological diagnosis, lack of coregistered pathology and images. Conclusions: nCLE in the pancreas is technically feasible via a 19-gauge needle under endosonographic guidance. Future studies will address identification of structures, diagnostic accuracy, and complication profiles. The rate of pancreatitis needs to be further clarified and mitigated.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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