Glutathione S Transferase P1, MI and TI Genotypes and Risk for Colorectal Cancer Development in Malaysian Population.
Background: Colorectal cancer (CRC) is a multifactorial disease with factors including dietary and lifestyle habits and genetic predisposition contributing to its etiopathogenesis. Even though the genetic predisposing factors are still unclear, genetic polymorphisms of genes encoding enzymes involve...
| Published in: | International Medical Journal Vol. 18; no. 4; pp. 279 - 283 |
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| Main Authors: | , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Japan International Cultural Exchange Foundation
Dec2011
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=71093480&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 71093480 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 13412051 23J1 jtl: International Medical Journal issn: 13412051 maglogo: N pubinfo: dt: Dec2011 vid: 18 iid: 4 pid: 29386 pub: Japan International Cultural Exchange Foundation artinfo: ui: 71093480 71093480 108154994 71093480 ppf: 279 ppct: 4 formats: fmt: @attributes: type: P tig: atl: Glutathione S Transferase P1, MI and TI Genotypes and Risk for Colorectal Cancer Development in Malaysian Population. aug: au: Mohd Shahpudin, Siti Nurfatimah Mustapha, Mohd Aminudin Abdul Aziz, Ahmad Aizat Murali Krishna, Bhavaraju Venkata Chatar Singh, Gurjeet Kaur Naik, Venkatesh R. Zakaria, Zaidi Mohd Sidek, Ahmad Shanwani Abu Hassan, Mohammad Radz Ankathi, Ravindran affil: Human Genome Centre, Universiti Sains Malaysia, Health Campus 16150 Kubang Kerian, Kelantan, Malaysia sug: subj: Colorectal Neoplasms Familial and Genetic Genotype Enzymes Glutathione Malaysia Human Funding Source Colorectal Neoplasms Physiopathology DNA Analysis Polymerase Chain Reaction Odds Ratio Confidence Intervals Case Control Studies Male Female Adult Middle Age Aged Descriptive Statistics Adult: 19-44 years Middle Aged: 45-64 years Aged: 65+ years Male Female ab: Background: Colorectal cancer (CRC) is a multifactorial disease with factors including dietary and lifestyle habits and genetic predisposition contributing to its etiopathogenesis. Even though the genetic predisposing factors are still unclear, genetic polymorphisms of genes encoding enzymes involved in xenobiotic metabolic pathways that activate or inactivate dietary carcinogens have been proposed as candidate genes. Three members of the Gluthathione S-Transferase (GSTs) family GSTPI, GSTT1 and GSTM1 have been analyzed in Malaysian population for polymorphic variants, and to elucidate their role in colorectal carcinogenesis. Objective: To determine the frequencies of GSTPI, GSTTI and GSTMI genotypes in 111 histopathologically confirmed CRC patients and 128 healthy controls and to evaluate the association risk of GSTPI, GSTTI and GSTMI genotypes on CRC predisposition. Material and Methods: Peripheral blood from the study subjects were collected in EDTA tubes and genomic DNA extracted using QIAGEN kit. The GSTPI IlelO5Val polymorphism was analyzed by PCR-RFLP technique using BsmAl restriction enzyme. The presence or absence of GSTM1 and GSTT1, genes were determined using a multiplex PCR protocol with albumin as the housekeeping gene. The resulting PCR fragments were separated on 2.0% agarose gel for GSTPI and 3.0% agarose gel electrophoresis for the GSTT1 and GSTM1. Results and Conclusion: On evaluating the CRC risk association with variant genotypes singly, GSTTI null genotype was associated significantly with an elevated risk (OR 1.804, 95%CI: 1.065-3.361, p=0027). When the polymorphic genotypes were analyzed in combination, the combination genotypes of GSTPI Val/Val/GSTM+/GSTTI÷ (OR 4.000), Val/Val/GSTMI-/GSTTI- (OR 3.000), and GSTPI Ile/Ile/GSTMI+/GSTTI- (OR 2.833) showed higher associated risk for CRC susceptibility, however the risk values were not statistically significant. Further studies involving larger sample size may help to identify the more specific risk groups and to determine factors of importance in CRC development. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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