Methicillin-resistant Staphylococcus aureus DNA electrophoretic pattern: temporal changes in an endemic hospital environment.
Objective. To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsed-field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil. Meth...
| Publicado en: | Pan American Journal of Public Health Vol. 30; no. 6; pp. 535 - 540 |
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| Autores principales: | , , |
| Formato: | Artículo |
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World Health Organization
Dec2011
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ssf&AN=71407666&site=ehost-live header: @attributes: shortDbName: ssf uiTerm: 71407666 longDbName: Social Sciences Full Text (H.W. Wilson) uiTag: AN controlInfo: bkinfo: jinfo: jid: 10204989 55OA jtl: Pan American Journal of Public Health issn: 10204989 maglogo: N pubinfo: dt: Dec2011 vid: 30 iid: 6 pid: 437 pub: World Health Organization artinfo: ui: 71407666 ppf: 535 ppct: 5 formats: fmt: @attributes: type: P size: 154KB tig: atl: Methicillin-resistant Staphylococcus aureus DNA electrophoretic pattern: temporal changes in an endemic hospital environment. aug: au: Leite, Gleice Cristina Padoveze, Maria Clara Moretti, Maria Luiza affil: Pós-Graduação em Clínica Médica, Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Sao Paulo, Brazil Departamento de Enfermagem em Saúde Coletiva, Escola de Enfermagem, Universidade de São Paulo, Sao Paulo, Brazil Departamento de Clínica Médica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Sao Paulo, Brazil su: Brazil DNA Pulsed-field gel electrophoresis Methicillin-resistant staphylococcus aureus Hospital patients sug: subj: Brazil DNA Pulsed-field gel electrophoresis Methicillin-resistant staphylococcus aureus Hospital patients keyword: drug resistance, microbial electrophoresis, gel, pulsed-field methicillin-resistant Staphylococcus aureus Staphylococcus aureus Brasil electroforesis en gel de campo pulsado farmacorresistencia microbiana Staphylococcus aureus Staphylococcus aureus resistente a meticilina drug resistance, microbial electrophoresis, gel, pulsed-field methicillin-resistant Staphylococcus aureus Staphylococcus aureus Brasil electroforesis en gel de campo pulsado farmacorresistencia microbiana Staphylococcus aureus Staphylococcus aureus resistente a meticilina ab: Objective. To describe the analysis of geographical and temporal distribution of DNA profiles determined by pulsed-field gel electrophoresis (PFGE) of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from hospitalized patients in a tertiary care university hospital in Brazil. Methods. Ninety-nine samples of MRSA obtained from 89 patients in the period 1999-2004 were studied. MRSA strains were isolated from central venous catheters (33 isolates) and bloodstream infections (66 strains). PFGE with 20 units of SmaI restriction endonuclease was used for genomic typing. Results. Analysis of DNA PFGE of 99 strains of MRSA revealed 26 profiles and their respective related profiles. The mean time interval for detecting MRSA infection was 26 days from hospital admission. Forty-nine patients (57.6%) had a recent hospitalization. The DNA PFGE MRSA profiles were distributed in three clonal groups--I, II, and III--according to the period of time when the MRSA strains were isolated. DNA PFGE MRSA profiles were spread homogeneously through all hospital wards. Conclusions. Changes in the distribution of DNA PFGE MRSA profiles were largely temporal, with clonal groups being replaced over time, without predominance in any hospital ward or any specific area of the hospital. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: N holdings: @attributes: islocal: N |
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