Cytotoxic effect of some natural compounds isolated from Lauraceae plants and synthetic derivatives.
Introduction. The antiproliferative effect of eleven neolignans, two lignans and one diterpene isolated from three Lauraceae plants, four benzofurans and two bicyclooctanes synthetic derivatives was evaluated in vitro on a set of five human cancer cells from solid tumors with a high incidence in Col...
| Published in: | Biomédica: Revista del Instituto Nacional de Salud Vol. 31; no. 3; pp. 335 - 344 |
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| Main Authors: | , , , , |
| Format: | Article |
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Instituto Nacional de Salud of Colombia
sep2011
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| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=lth&AN=71927794&site=ehost-live header: @attributes: shortDbName: lth uiTerm: 71927794 longDbName: MedicLatina uiTag: AN controlInfo: bkinfo: jinfo: jid: 01204157 3ANP jtl: Biomédica: Revista del Instituto Nacional de Salud issn: 01204157 maglogo: N pubinfo: dt: sep2011 vid: 31 iid: 3 pid: 35431 pub: Instituto Nacional de Salud of Colombia artinfo: ui: 71927794 ppf: 335 ppct: 9 formats: fmt: @attributes: type: P size: 5.6MB tig: atl: Cytotoxic effect of some natural compounds isolated from Lauraceae plants and synthetic derivatives. aug: au: Enrique Cuca, Luis Coy, Ericsson David Alarcón, Marlén Andrea Fernández, Andrés Ancízar Aristizáábal, Fabio affil: Laboratorio de Investigación en Productos Naturales Vegetales, Departamento de Química, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia Laboratorio de Farmacogenética del Cáncer, Departamento de Farmacia, Facultad de Ciencias, Universidad Nacional de Colombia, Bogotá, D.C., Colombia su: Lignans Diterpenes Terpenes Lauraceae Benzofurans sug: subj: Lignans Diterpenes Terpenes Lauraceae Benzofurans keyword: biological products diterpenes in vitro straining cribado diterpenos in vitro Lauraceae productos biológicos ab: Introduction. The antiproliferative effect of eleven neolignans, two lignans and one diterpene isolated from three Lauraceae plants, four benzofurans and two bicyclooctanes synthetic derivatives was evaluated in vitro on a set of five human cancer cells from solid tumors with a high incidence in Colombia. Objective. To evaluate the cytotoxic effect of twenty compounds on the tumor cell lines HeLa, A-549. Hep-2, PC-3, and MCF-7. Materials and methods. Fourteen natural compounds were isolated by chromatographic techniques from three native colombian plants (Pleurothyrium cinereum, Ocotea macrophylla and Nectandra amazonum), whose structures were established by spectroscopic methods; six synthetic derivatives were prepared by oxyarylation and diazomethane methylation. Antiproliferative effect and cell recovery were performed by means of in vitro treatment of tumor cell lines with test compounds, evaluating cell viability by resazurin staining. Results. Among test compounds, only neolignans ocophyllal A, cinerin D, kaurenoic acid, two benzofuran-derivatives, and synthetic (-)-cinerin A were found to have antiproliferative effect at different levels. Bicyclooctanoids as well as kaurenoic acid exhibited activity against all human cancer cells while benzofuranoids showed selective activity against HeLa. Furthermore, compounds (-)-cinerin A and kaurenoic acid exhibited total lethal effect against all-five cell lines and PC-3, Hep-2, and A549 cell lines, respectively. Conclusion. Test compounds exhibiting antiproliferative activity showed interesting results, which would promote their use as lead compounds on further studies for anticancer agents development. Introducción. El efecto contra la proliferación celular de once neolignanos, dos lignanos y un diterpeno, aislados de tres plantas de la familia Lauraceae, y cuatro benzofuranos y dos biciclooctanos sintéticos, fue evaluado in vitro sobre cinco líneas celulares derivadas de tumores sólidos de alta incidencia e Colombia. Objetivo. Evaluar el efecto citotóxico de veinte compuestos sobre las líneas tumorales HeLa, A-549, Hep-2, PC-3 y MCF-7. Materiales y metodos. Los 14 compuestos de origen natural fueron aislados de tres plantas nativas colombianas (Pleurothyrium cinereum, Ocotea macrophylla y Nectandra amazonum) por técnicas cromatográficas y se establecieron sus estructuras por métodos espectroscóópicos, y los seis derivados sintéticos fueron preparados mediante reacción de oxiarilación y metilación con diazometano. El efecto contra la proliferación y la recuperación celular se hicieron mediante tratamiento in vitro de las líneas tumorales con los compuestos, evaluando la viabilidad celular por tinción con resazurina. Resultados. Entre los compuestos evaluados, solamente ocofilal A, cinerina D, ácido kaurenoico, dos benzofuranos y la (-)-cinerina A sintéética presentaron actividad contra la proliferación celular en diferentes niveles. Los biciclooctanos, asi como el ácido kaurenoico, fueron activos contra todas las líneas celulares, mientras que los benzofuranoides mostraron actividad selectiva contra HeLa. Además, la (-)-cinerina A exhibió un efecto letal total contra todas las líneas celulares, mientras que el ácido kaurenóico presentó efecto letal total contra PC-3, Hep-2 y A549. Conclusion. Los compuestos evaluados que exhibieron actividad contra la proliferación celula mostraron resultados interesantes, Io cual sugiere su potencial uso como cabezas de serie o moléculas plantilla en el desarrollo de agentes anticancerígenos. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: Y custom: Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites without the copyright holder's express written permission. Additionally, content may not be used with any artificial intelligence tools or machine learning technologies. However, users may print, download, or email articles for individual use. item: Biomédica: Revista del Instituto Nacional de Salud holder: Instituto Nacional de Salud of Colombia dt: @attributes: year: 2011 holdings: @attributes: islocal: N |
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