Alternate site testing for HbA1c using the Primus CLC330 GHb analyzer.
Objective: To determine whether the Primus high-pressure liquid chromatography (HPLC) is suited to alternate site testing (AST) for HbA1c in a hospital diabetes outpatient clinic.Research Design and Methods: Patients were attending the clinic for routine management of their diabetes. A number of dia...
| Publicado en: | Diabetes Care Vol. 20; no. 4; pp. 607 - 610 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American Diabetes Association
Apr1997
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=72280461&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 72280461 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 01495992 1G7 jtl: Diabetes Care issn: 01495992 maglogo: N pubinfo: dt: Apr1997 vid: 20 iid: 4 pid: 1367 pub: American Diabetes Association place: Arlington, Virginia artinfo: ui: 72280461 72280461 NLM9096987 72280461 10.2337/diacare.20.4.607 NLM9096987 72280461 ppf: 607 ppct: 3 formats: tig: atl: Alternate site testing for HbA1c using the Primus CLC330 GHb analyzer. aug: au: Phillipov, George Charles, Peter Beng, Clive Phillips, Patrick J. Phillipov, G Charles, P Beng, C Phillips, P J affil: Endocrine and Diabetes Service, The Queen Elizabeth Hospital, Woodville, Australia sug: subj: Diabetes Mellitus Blood Chromatography, High Pressure Liquid Equipment and Supplies Chromatography, Liquid Methods Chromatography, High Pressure Liquid Methods Human Chromatography, Ion Exchange Methods Sensitivity and Specificity Time Factors Reproducibility of Results Validation Studies Comparative Studies Evaluation Research Multicenter Studies Ferrans and Powers Quality of Life Index ab: Objective: To determine whether the Primus high-pressure liquid chromatography (HPLC) is suited to alternate site testing (AST) for HbA1c in a hospital diabetes outpatient clinic.Research Design and Methods: Patients were attending the clinic for routine management of their diabetes. A number of diabetic patients with uremia (n = 11) were also investigated. HbA1c levels were measured in the outpatient setting by the Primus HPLC and in a more limited study the DCA-2000 instrument using the new 6-min assay cartridge. HbA1c measurements were also performed with Pierce affinity minicolumns and a Bio-Rad Variant HPLC.Results: The Primus HPLC assay had low imprecision of 2.3, 1.6, and 1.0% for HbA1c levels of 4.7, 7.3, and 11.1%, respectively, and was not prone to interference by carbamylated hemoglobin as found for the ion-exchange Variant HPLC method. Method comparison studies showed that the bias and proportional error between the Pierce affinity minicolumn procedure (standardized with respect to an external quality control program) and the Primus HPLC (Y) was -0.4 and 1.2% respectively (n = 32). Similarly the bias and proportional error between the Primus and DCA-2000 methods was 0.7 and -2.5%. The Primus was shown to give falsely elevated HbA1c concentrations if the time between sequential injections was > 28 min.Conclusions: The Primus HPLC has a decided advantage over specialty AST instruments, like the DCA-2000, in not only meeting AST requirements but also allowing rapid automated batch processing of all laboratory HbA1c samples. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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