Rolling Circle Amplification-Templated DNA Nanotubes Show Increased Stability and Cell Penetration Ability.

DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results...

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Publicado en:Journal of the American Chemical Society Vol. 134; no. 6; pp. 2888 - 2892
Autores principales: Hamblin, Graham D., Carneiro, Karma M. M., Fakhoury, Johans F., Bujold, Katherine E., Sleiman, Hanadi F.
Formato: Artículo
Publicado: American Chemical Society 2/15/2012
Materias:
Acceso en línea:Ver este registro en EBSCOhost
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      pub: American Chemical Society
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        10.1021/ja2107492
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        atl: Rolling Circle Amplification-Templated DNA Nanotubes Show Increased Stability and Cell Penetration Ability.
      aug:
        au:
          Hamblin, Graham D.
          Carneiro, Karma M. M.
          Fakhoury, Johans F.
          Bujold, Katherine E.
          Sleiman, Hanadi F.
        affil: Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC H3A 2K6 Canada
      su:
        DNA machinery
        Nanostructured materials synthesis
        Nanotubes
        Gene amplification
        Drug delivery devices
        Nucleic acid probes
        Imaging systems
      sug:
        subj:
          DNA machinery
          Nanostructured materials synthesis
          Nanotubes
          Gene amplification
          Drug delivery devices
          Nucleic acid probes
          Imaging systems
      ab: DNA nanotubes hold promise as scaffolds for protein organization, as templates of nanowires and photonic systems, and as drug delivery vehicles. We present a new DNA-economic strategy for the construction of DNA nanotubes with a backbone produced by rolling circle amplification (RCA), which results in increased stability and templated length. These nanotubes are more resistant to nuclease degradation, capable of entering human cervical cancer (HeLa) cells with significantly increased uptake over double-stranded DNA, and are amenable to encapsulation and release behavior. As such, they represent a potentially unique platform for the development of cell probes, drug delivery, and imaging tools.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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