Expression of a specific subset of CD44 variant transcripts in NOD pancreatic islets.
Adhesion of lymphocytes to the endothelial venules inside the islets of Langerhans seems to initiate the infiltration of islets in NOD mice. An overexpression of the lymphocyte surface molecule CD44 in infiltrated NOD islets compared with peripheral blood lymphocytes was recently reported. The CD44...
| Publicado en: | Diabetes Vol. 45; no. 6; pp. 718 - 725 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
American Diabetes Association
Jun1996
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=73510436&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 73510436 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00121797 1G6 jtl: Diabetes issn: 00121797 maglogo: N pubinfo: dt: Jun1996 vid: 45 iid: 6 pid: 1367 pub: American Diabetes Association place: Arlington, Virginia artinfo: ui: 73510436 73510436 NLM8635643 73510436 10.2337/diab.45.6.718 NLM8635643 73510436 ppf: 718 ppct: 7 formats: tig: atl: Expression of a specific subset of CD44 variant transcripts in NOD pancreatic islets. aug: au: Milde, Kerstin F. Alonso, Miriam Shen Shen Kong Alejandro, Rodolfo Mintz, Daniel H. Pastori, Ricardo L. Milde, K F Alonso, M Kong, S S Alejandro, R Mintz, D H Pastori, R L affil: Diabetes Research Institute, University of Miami School of Medicine, Miami, Florida sug: subj: Diabetes Mellitus, Type 1 Immunology Islets of Langerhans Immunology Genetics RNA Polymerase Chain Reaction Mice Diabetes Mellitus, Type 1 Nucleotides DNA Probes Female Animal Studies Documentation Validation Studies Comparative Studies Evaluation Research Multicenter Studies Interview Guides Female ab: Adhesion of lymphocytes to the endothelial venules inside the islets of Langerhans seems to initiate the infiltration of islets in NOD mice. An overexpression of the lymphocyte surface molecule CD44 in infiltrated NOD islets compared with peripheral blood lymphocytes was recently reported. The CD44 protein family includes a variety of molecules generated by alternative RNA splicing from 10 variant exons (v1-v10). By using reverse transcriptase-polymerase chain reaction followed by Southern blotting and hybridization to exon-specific cDNA probes, we investigated the expression of CD44 isoforms in highly purified islets of Langerhans from 4- and 10-week-old NOD mice. At least six CD44 isoforms were strongly overexpressed in NOD islets at 4 and 10 weeks when compared with age-matched BALB/c islets. Controls in different tissues indicate that these variants are specifically increased in the islets from the NOD strain. Islets from the NOD-scid/scid strain also expressed these variant exons. Splenocytes from BALB/c did not express CD44 isoforms, whereas splenocytes from 4-week-old NOD mice did express CD44 variants. Treatment with inflammatory mediators induced new isoforms; however, these transcripts have a different variant exon composition from that found in NOD mice islets. These results suggest that some isoforms are expressed very early in the development of insulitis by a component of the NOD islet itself and underscore a possible role of CD44 in islet infiltration. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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