Development of NIR Fluorescent Dyes Based on Si-rhodamine for in Vivo Imaging.

We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si—rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Bo...

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Publicado en:Journal of the American Chemical Society Vol. 134; no. 11; pp. 5029 - 5032
Autores principales: Koide, Yuichiro, Urano, Yasuteru, Hanaoka, Kenjiro, Wen Piao, Kusakabe, Moriaki, Saito, Nae, Terai, Takuya, Okabe, Takayoshi, Nagano, Tetsuo
Formato: Artículo
Publicado: American Chemical Society 3/21/2012
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Acceso en línea:Ver este registro en EBSCOhost
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      dt: 3/21/2012
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      pub: American Chemical Society
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        10.1021/ja210375e
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        atl: Development of NIR Fluorescent Dyes Based on Si-rhodamine for in Vivo Imaging.
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        au:
          Koide, Yuichiro
          Urano, Yasuteru
          Hanaoka, Kenjiro
          Wen Piao
          Kusakabe, Moriaki
          Saito, Nae
          Terai, Takuya
          Okabe, Takayoshi
          Nagano, Tetsuo
        affil:
          Graduate School of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
          CREST, JST, Japan Science and Technology Agency, 3-5 Sanbancho, Chiyoda, Tokyo 102-0075, Japan
          Medicine, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
          Agricultural and Life Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
          Matrix Cell Research Institute, Inc., Ushiku, Ibaraki, 300-1232, Japan
          Open Innovation Center for Drug Discovery, University of Tokyo, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan
      su:
        Fluorescent dyes
        Chemical structure
        Fluorescent dyes spectra
        Silicon compounds
        Rhodamines
        Imaging systems
        Fluorescent probes
      sug:
        subj:
          Fluorescent dyes
          Chemical structure
          Fluorescent dyes spectra
          Silicon compounds
          Rhodamines
          Imaging systems
          Fluorescent probes
      ab: We have developed a series of novel near-infrared (NIR) wavelength-excitable fluorescent dyes, SiR-NIRs, by modifying the Si—rhodamine scaffold to obtain emission in the range suitable for in vivo imaging. Among them, SiR680 and SiR700 showed sufficiently high quantum efficiency in aqueous media. Both antibody-bound and free dye exhibited high tolerance to photobleaching in aqueous solution. Subcutaneous xenograft tumors were successfully visualized in a mouse tumor model using SiR700-labeled anti-tenascin-C (TN-C) antibody, SiR700-RCB1. SiR-NIRs are expected to be useful as labeling agents for in vivo imaging studies including multicolor imaging, and also as scaffolds for NIR fluorescence probes.
      pubtype: Academic Journal
      doctype: Article
      src: R
    language: English
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          year: 2012
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