A broad-spectrum analysis of the effects of teflubenzuron exposure on the biochemical activities and microbial community structure of soil
We evaluated the response of soil bacteria to applications of the insecticide teflubenzuron at the field rate dosage (FR; 0.15 mg/kg of soil) and at a higher dosage (10*FR; 1.5 mg/kg of soil). When applied at the FR dosage, teflubenzuron had no effect on several biochemical parameters of the soil, i...
| Published in: | Journal of Environmental Management Vol. 108; pp. 27 - 36 |
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| Main Authors: | , , , |
| Format: | Article |
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Academic Press Inc.
Oct2012
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| Subjects: | |
| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ssf&AN=77758733&site=ehost-live header: @attributes: shortDbName: ssf uiTerm: 77758733 longDbName: Social Sciences Full Text (H.W. Wilson) uiTag: AN controlInfo: bkinfo: jinfo: jid: 03014797 EMJ jtl: Journal of Environmental Management issn: 03014797 maglogo: N pubinfo: dt: Oct2012 vid: 108 pid: 735 pub: Academic Press Inc. artinfo: ui: 77758733 10.1016/j.jenvman.2012.05.002 ppf: 27 ppct: 9 formats: tig: atl: A broad-spectrum analysis of the effects of teflubenzuron exposure on the biochemical activities and microbial community structure of soil aug: au: Cycoń, Mariusz Wójcik, Marcin Borymski, Sławomir Piotrowska-Seget, Zofia affil: Department of Microbiology and Virology, Medical University of Silesia, Jagiellońska 4, 41-200 Sosnowiec, Poland Department of Ecotoxicology, Institute of Industrial Organic Chemistry, Doświadczalna 27, 43-200 Pszczyna, Poland Department of Microbiology, University of Silesia, Jagiellońska 28, 40-032 Katowice, Poland su: Population dynamics Soil microbiology Teflubenzuron Toxicology of insecticides Soil pesticide content measurement Dose-response relationship in biochemistry Effect of insecticides on non-target organisms Microorganism populations Biochemical toxicology sug: subj: Population dynamics Soil microbiology Teflubenzuron Toxicology of insecticides Soil pesticide content measurement Dose-response relationship in biochemistry Effect of insecticides on non-target organisms Microorganism populations Biochemical toxicology keyword: Biodegradation Enzyme activities Microbial biodiversity Nitrogen transformation PLFA Biodegradation Enzyme activities Microbial biodiversity Nitrogen transformation PLFA ab: We evaluated the response of soil bacteria to applications of the insecticide teflubenzuron at the field rate dosage (FR; 0.15 mg/kg of soil) and at a higher dosage (10*FR; 1.5 mg/kg of soil). When applied at the FR dosage, teflubenzuron had no effect on several biochemical parameters of the soil, including substrate-induced respiration (SIR), dehydrogenase (DHA) and phosphatase activities (PHOS), and N–NO and N–NH concentrations. Additionally, no differences were observed in the culturable fraction of the soil bacteria (the number of heterotrophic, nitrifying, denitrifying and N-fixing bacteria; the growth strategy; the ecophysiological and colony development indices; and the physiological state). In contrast, treatment with the 10*FR dosage of the insecticide significantly increased SIR, DHA, PHOS and N–NH levels and the number of heterotrophic and denitrifying bacteria. Decreases in urease activity (URE) and the number of nitrifying and N-fixing bacteria were also observed. A phospholipid fatty acid (PLFA) method-based analysis of the entire soil microorganism population revealed that teflubenzuron treatment affected the total fatty acid level as well as those considered to be of Gram-positive bacteria, Gram-negative bacteria and fungi. This effect was observed on days 1 and 14 post-treatment. A principal component analysis (PCA) of the PLFAs showed that teflubenzuron treatment significantly shifted the microbial community structure; however, all of the observed effects were transient. Studies on the degradation of teflubenzuron revealed that this process is characterised by a short lag phase and a rate constant (k) of 0.020/day. This degradation rate follows first-order kinetics, and the DT50 was 33.5 days. This is the first study that thoroughly examines the functional and structural status of both the culturable and non-culturable fractions of the soil microbial community after teflubenzuron application. pubtype: Academic Journal doctype: Article src: R language: English refInfo: copyright: @attributes: flag: N holdings: @attributes: islocal: N |
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